胶束毛细管电泳在线推扫技术分离检测猪肉组织中痕量喹诺酮类药物

利用胶束毛细管电泳法结合在线推扫富集技术对组织中残留的痕量环丙沙星、氧氟沙星和恩诺沙星进行了检测, 弥补了毛细管电泳检测灵敏度低的缺点, 大大减化了操作过程, 为动物食品组织中残留的痕量药物检测提供了一种新的简便可靠的方法.

On-line Sweeping Determination of Quinolones in Pork Tissue by Micellar Capillary Electrophoresis

A micellar capillary electrophoresis(MCE) method based on on-line sweeping technique was developed for a determination of trace quinolones, ciprofloxacin ofloxacin and enrofloxacin in pork tissue. The pork tissue samples were deproteinized with trichloroacetic acid and in the running buffer of 20 mmol/L Na2B4O7+80 mmol/L sodium dodecyl sulfate(SDS), pH=9.6, the ciprofloxacin, ofloxacin and enrofloxacin in the sample were successfully separated and detected within 200 s, in a non-coating fused-silica capillary column(48.5 cm×75 μm i.d., effective length 40 cm) at separation voltage of 18 kV, and the injection pressure of 15×102 Pa, with UV detection at 278 nm. The detection limits of ciprofloxacin, ofloxacin and enrofloxacin were 0.007, 0.016 and 0.020 mg/L, and RSD were 2.0%, 2.4% and 2.6%, respectively. This method allows of up to 600-fold accumulation concentration of ciprofloxacin and can be used to separate and detect the residuals of trace medicines in animal food.