| 细胞色素c氧化酶可溶性结构域中Val162突变对蛋白结构和功能的影响 |
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| 引用本文： | 宋爱新,李连之,黄仲贤.细胞色素c氧化酶可溶性结构域中Val162突变对蛋白结构和功能的影响[J].高等学校化学学报,2003,24(10):1733-1737. |
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| 作者姓名： | 宋爱新 李连之 黄仲贤 |
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| 作者单位： | 复旦大学化学系, 化学生物学实验室, 上海 200433 |
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| 基金项目： | 国家自然科学基金，39990600， |
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| 摘 要： | 采用蛋白质工程的定点突变技术和UV-Vis,CD,荧光,EPR及快速动力学技术考察了与癌症相关的Val162的突变对细胞色素c氧化酶可溶性结构域的影响.研究发现,该突变导致了蛋白二级结构的转化和电子传递活性的降低,这为理解其致病机制提供了化学基础信息.
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| 关 键 词： | 细胞色素c氧化酶 点突变与疾病 结构转化 电子传递 |
| 文章编号： | 0251-0790(2003)10-1733-05 |
| 收稿时间： | 2002-12-18 |
Effect of Val162 Mutation on Structure and Function of Cytochrome c Oxidase Soluble Domain |
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| SONG Ai-Xin,LI Lian-Zhi,HUANG Zhong-Xian.Effect of Val162 Mutation on Structure and Function of Cytochrome c Oxidase Soluble Domain[J].Chemical Research In Chinese Universities,2003,24(10):1733-1737. |
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| Authors: | SONG Ai-Xin LI Lian-Zhi HUANG Zhong-Xian |
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| Institution: | Chemistry and Biology Lab, Department of Chemistry, Fudan University, Shanghai 200433, China |
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| Abstract: | The effect of Val162 mutation(potentially relative to cancer) on cytochrome c oxidase soluble Cu A domain was studied by site-directed mutagenesis technique combined with UV-Vis, CD, fluorescence spectra, EPR and rapid kinetic methods. It is found that the mutation at the Val162 site leads to a structural transformation and decreasing electron transfer activity. It provides important information for understanding the pathological mechanism. |
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| Keywords: | Cytochrome c oxidase Site-directed mutation and diseases Structure transformation Electron transfer |
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