质膜微囊的分离及原子力显微镜研究

采用非去污剂在OptiPrep梯度中漂浮超声处理质膜的方法, 从鼠肺中分离质膜微囊, 在原子力显微镜下以不同条件进行了观察. 该非去污剂法能分离出完整的质膜微囊, 在去离子水稀释500倍的条件下得到了清晰的质膜微囊的原子力显微镜图片, 建立了一种新的快速分离完整质膜微囊的方法和用原子力显微镜观测质膜微囊结构的新方法.

Separation of Caveolae and Atomic Force Microscopy Study of Them

Caveolae were spherical,elliptical or flask-shaped invaginations(50-100 nm in diameter) on the surface of cell plasma membrane.They play important roles in many biological processes,such as endocytosis,pinocytosis,cholesterol transport,signal transduction,internalization of bacteria and viruses,and so on.In this research,caveolae were separated from rat lungs by floating plasma membrane treated with ultrasonic in OptiPrep gradient(a detergent-free method),and were observed by atomic force microscopy(AFM) under different conditions.By using this method,intact caveolae were isolated from plasma membrane,and the best AFM images of caveolae were obtained by diluting them 500 times with deionized water.So,a new method for separating caveolae rapidly and completely was established,and a new way to observe the structures of caveolae was set up.