电化学诱导咪唑铜配合物断裂DNA的研究

二价咪唑铜配合物可以在电极表面发生氧化还原反应,该过程对应于单电子准可逆过程.荧光光谱电化学结果表明,还原产物一价咪唑铜配合物可以被空气中的O₂氧化为二价.在中性的Tris-HCl缓冲溶液中,配合物与DNA之间存在弱相互作用,其键合常数仅为2.7×10²L/mol.荧光光谱及电化学结果表明为嵌入式作用.据此建立了一种电化学诱导咪唑铜配合物对DNA断裂的新方法,并采用差示扫描量热(DSC)法检验了断裂结果.

Studies on Electrochemically Induced DNA Cleavage by Imidazole Copper Complex

There are a couple of quasi-reversible redox peaks of Cu(Ⅱ)-imidazoleCu(Ⅱ)Im₄] with one electron process on Pt electrode. Fluorescence spectroelectrochemical experiments reveal that the Cu(Ⅰ) complex reduced by electrochemistry can be reoxidized by O₂ in air. In neutral Tris-HCl buffer, {Cu(Ⅱ)Im₄} can bind to DNA with the binding constant of 2.7×10 2 L/mol. Obviously, this is a weak interaction. However, it is an intercalative model which has been identified by the experiments of fluorescence and electrochemistry. A novel method for the cleavage of DNA by the electrochemically-induced process of the reduction of Cu(Ⅱ)Im₄ is established and differential scanning calorimetries(DSC) are used to detect the results of DNA cleavage.