反胶束体系中脂肪酶催化合成异丁酸异戊酯

报道了在CTAB/正己烷和AOT/正己烷反胶束体系中,CCL脂肪酶催化合成异丁酸异戊酯的新方法.考察了水含量w0、底物与酶的比例、缓冲溶液pH值以及温度等因素对脂肪酶催化酯合成反应的影响.研究结果表明,两种反胶束体系均为合成异丁酸异戊酯提供了较为合适的微环境,所选定的脂肪酶在CTAB和AOT反胶束中的活性分别是有机溶剂中反应活性的6倍和4倍.

Lipase-catalyzed Synthesis of Isoamyl Isobutyrate in Reversed Micelle System

A new method of lipase-catalyzed esterification of isoamyl isobutyrate by CCL in the reversed micelle of CTAB/n-hexane and AOT/n-hexane has been reported. The effects of such factors as water content(w 0), ratio of substrate to enzyme, pH of buffer and temperature on the synthesis of esterification were investigated. Experimental results show that the reaction rate of esterification in the system of CTAB/n-hexane is faster than that in the system of AOT/n-hexane, and that the conversion of esterification reaches a climax of 99% in the two systems in 80 h. Both the two kinds of reversed micelle provide a suitable micro-environment for the selected lipase(CCL), which shows superactivity 6 and 4 times as much as that in organic solvent, respectively.