| 糖的基质辅助激光解吸/电离质谱(MALDI-MS)研究 |
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| 引用本文： | 郝春雁,马秀俐,刘志强,季怡萍,刘淑莹,刘长城,孙允秀,刘举正.糖的基质辅助激光解吸/电离质谱(MALDI-MS)研究[J].高等学校化学学报,1998,19(7):1090-1094. |
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| 作者姓名： | 郝春雁 马秀俐 刘志强 季怡萍 刘淑莹 刘长城 孙允秀 刘举正 |
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| 作者单位： | 1. 中国科学院长春应用化学研究所, 长春, 130022;
2. 吉林大学化学系, 长春, 130023 |
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| 摘 要： | 通过糖类化合物3种常用基质MALDI-MS分析效果的比较以及寡糖和多糖正、负离子MALDI-MS谱的对比,找到了适合糖分析的基质2,5-DHB,探讨了糖类化合物激光解吸/电离条件下形成离子的过程,指出了Na+、K+离子在寡糖分子量测定中的重要作用,借助柱层析分离手段,成功地测出了分子量大于10000的葡聚糖的分子量。
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| 关 键 词： | 基质辅助激光解吸/电离质谱(MALDI-MS) 寡糖 多糖 分子量 |
| 收稿时间： | 1997-06-24 |
| 修稿时间： | 1997年6月24日 |
Matrixassisted Laser Desorption/Ionization Mass Spectral Study of Saccharides |
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| HAO Chun-Yan,MA Xiu-Li,LIU Zhi-Qiang,JI Yi-Ping,LIU Shu-Ying,LIU Chang-Cheng,SUN Yun-Xiu,LIU Ju-Zheng.Matrixassisted Laser Desorption/Ionization Mass Spectral Study of Saccharides[J].Chemical Research In Chinese Universities,1998,19(7):1090-1094. |
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| Authors: | HAO Chun-Yan MA Xiu-Li LIU Zhi-Qiang JI Yi-Ping LIU Shu-Ying LIU Chang-Cheng SUN Yun-Xiu LIU Ju-Zheng |
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| Institution: | 1. ChangChun Institute of AppLied Chemistry, A caden is Sinica, ChangChun, 130022;
2. Dep of Chan., JiLin Universilyty, Changchun, 130023 |
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| Abstract: | Matrixassisted laser desorption/ionization (MALDI) mass spectra of saccharides with three common matrixes were studied here. It is shown that Na + and K + play important roles in the determination of oligasaccharide molecule weight and the molecular weights(MW) of glucans(dextran) with MW more than 10 000 is determined successfully with the help of column chromatography. By comparing the effects of three matrixes in saccharide analysis and comparing positive ion and negative ion matrixassisted laser desorption/ionization mass spectra of oligo and polysaccharides, the most suitable matrix for saccharide analysis, 2,5 dihydroxylbezonic acid (DHB), is selected and the ion formation processes of saccharides under matrix assisted laser adsorption/ionization condition are clarified. |
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| Keywords: | Matrixassisted laser desorption/ionization mass spectrometry Oligosaccharide Polysaccharide Molecular weight |
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