反应性梳状聚乙二醇改性聚酯薄膜表面及其内皮细胞相容性的研究

以高密度梳状PEG(CPEG)作为表面改性材料, 将PEG末端羟基转化为醛基, 将梳状PEG和线形PEG固定在氨基化的PET膜表面, 并利用表面的反应性醛基进一步固定了氨基酸和整合素配体多肽片段RGD多肽. 红外光谱、 接触角和X射线光电子能谱(XPS)测定结果表明, 该法可有效地固定氨基酸和多肽, 获得模拟细胞膜中多糖-蛋白质复合物结构的特异性功能表面. 对两种不同结构的PEG细胞培养实验结果表明, CPEG比线形PEG(LPEG)具有更好的抗非特异粘附性. 此外, CPEG比LPEG具有更多的活性反应基团, 用PEG末端活性的醛基固定整合素配体多肽片段RGD, 可有效地诱导材料表面的内皮细胞化, 改善材料的细胞相容性.

Surface Tailoring of PET via Immobilization of Comb-shaped Poly(ethylene glycol) for Promoting Endothelial Cell Compatibility

The hydroxyl end groups of a comb-like(poly(ethylene) glycol) were transformed into aldehyde groups,which were further grafted onto PET substrate.Ligands including lysine and RGD peptide were tethered onto the reactive PEG end groups.Linear PEGs with different molecule weights(M_w=4 000 and 10 000) were also investigated as controls.The surface grafting process was characterized by means of FTIR,()~(1)H NMR,contact angle and XPS.The two systems were compared with regarding their abilities to prevent non-specific interaction and to promote cyto-compatibility.Owing to its high branched-arm structure,the comb-like PEG modified surface(achieved) stronger entropic repulsion than the linear PEG.Meanwhile,more ligands were tethered onto the comb-like PEG modified surface with a great amount of reactive end groups,which increased cell proliferation.The glycocalyx mimicking surface designed with comb-shaped PEG end-tethered by RGD peptide was proved to present non-specific resistance and excellent cyto-compatibility simultaneously.