| 用于体外基因转染的氨基硅烷Fe3O4复合纳米粒子的合成及表征 |
| |
| 引用本文： | 李文章,李洁,谭泽明.用于体外基因转染的氨基硅烷Fe3O4复合纳米粒子的合成及表征[J].高等学校化学学报,2009,30(12):2331-2336. |
| |
| 作者姓名： | 李文章 李洁 谭泽明 |
| |
| 作者单位： | 1. 中南大学化学化工学院,长沙,410083
2. 中南大学湘雅医院神经外科,长沙,410078 |
| |
| 基金项目： | 教育部新世纪优秀人才资助计划，湖南省重大科技计划项目 |
| |
| 摘 要： | 利用2-吡咯烷酮和乙酰丙酮铁为原料制备Fe3O4磁性纳米颗粒, 用XRD和TEM对样品进行了表征. 选择偶联剂γ-氨丙基三乙氧基硅烷NH2C3H6Si(OC2H5)3]对纳米粒子进行表面修饰, 制得APTTS/Fe3O4复合载体材料. 以此复合粒子作为传递载体, 将CD基因转染U251胶质瘤细胞. 采用RT-PCR, Western blot及免疫荧光等方法检测CD基因的表达及功能. 结果表明, 制备的Fe3O4颗粒粒径为8~10 nm, 结晶度较高; 经表面修饰后, 粒子表面负载—OH, —NH, —NH2, —C—O和—C—OH等多种功能基团. DNA结合分析及DNase-I消化结果表明, APTTS/Fe3O4粒子能够有效地结合和保护DNA. 体外细胞转染实验证实, 该复合纳米颗粒能够高效地传递CD基因进入U251胶质瘤细胞内, 并进行稳定表达.
|
| 关 键 词： | 纳米四氧化三铁 磁性材料 体外转染 基因载体 |
| 收稿时间： | 2009-03-06 |
Synthesis and Characterization of APTTS-Fe_3O_4 Nanoparticles for in vitro Transfection as Gene Carriers |
| |
| LI Wen-Zhang,LI Jie,TAN Ze-Ming.Synthesis and Characterization of APTTS-Fe_3O_4 Nanoparticles for in vitro Transfection as Gene Carriers[J].Chemical Research In Chinese Universities,2009,30(12):2331-2336. |
| |
| Authors: | LI Wen-Zhang LI Jie TAN Ze-Ming |
| |
| Institution: | LI Wen-Zhang1,LI Jie1,TAN Ze-Ming2(1.College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China,2.Neurosurgery Department of Xiang-Ya Hospital,Changsha 410078,China) |
| |
| Abstract: | Fe3O4 magnetic nanoparticles were prepared via thermal decomposition of Fe(acac)3 in 2-pyrrolidone.And 3-aminopropyltriethoxy-silane(APTTS)was used to modify the surface of the nanoparticles.The structure and properties of the samples were characterized by means of XRD,TEM and FTIR.The potency of adsorbing DNA and resisting DNase-I digestion of APTTS/Fe3O4 were analyzed via agarose gel electrophoresis.Then,APTTS/Fe3O4 nanoparticles were evaluated as a kind of plasmid pCMVCD carrier and transferred into huma... |
| |
| Keywords: | Fe_3O_4 nanoparticle Magnetic material In vitro transfection Gene carrier |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《高等学校化学学报》浏览原始摘要信息 |
| 点击此处可从《高等学校化学学报》下载免费的PDF全文 |
|
