| 螺旋藻糖蛋白的分离纯化及其性质研究 |
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| 引用本文： | 殷钢,刘铮,李琛,刘飞,丁富新,袁乃驹.螺旋藻糖蛋白的分离纯化及其性质研究[J].高等学校化学学报,1999,20(4):565-568. |
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| 作者姓名： | 殷钢 刘铮 李琛 刘飞 丁富新 袁乃驹 |
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| 作者单位： | 清华大学化学工程系,北京,100084 |
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| 基金项目： | 国家“九·五”科技攻关项目 |
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| 摘 要： | 采用新的分离工艺从螺旋藻中提取得到粗多糖经环流泡沫分离去除蛋白和ＳｅｐｈａｃｒｙｌＳ－１００柱层析纯化后得到一种糖蛋白。经ＳｅｈｐｈａｄｅｘＧ－２００和和凝胶电证实所得的糖蛋白为均一组成；
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| 关 键 词： | 螺旋藻 糖蛋白 多糖 |
Isolation, Characterization and Properties of Spirulina Glycoprotein |
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| YIN Gang,LIU Zheng,LI Chen,LIU Fei,DING Fu-Xin,YUAN Nai-Ju.Isolation, Characterization and Properties of Spirulina Glycoprotein[J].Chemical Research In Chinese Universities,1999,20(4):565-568. |
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| Authors: | YIN Gang LIU Zheng LI Chen LIU Fei DING Fu-Xin YUAN Nai-Ju |
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| Abstract: | A glycoprotein was isolated from Chinese spirulina. Purification of this glycoprotein was accomplished by foam in a loop bubble column and gel chromatography on Sephacryl S100. The homogeneity of this glycoprotein was proven by gel filtration on Sephadex G200 and SDSPAGE. The molecular weight of the glycoprotein was \{130 000\}. Characterization of this glycoprotein by IR analysis showed the typical absorption of polysaccharide and indicated the presence of glycoside linkage. Elimination reaction demonstrated the existence of Oglycosidic linkage in this spirulina glycoprotein. |
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| Keywords: | Spirulina Glycoprotein Polysaccharide |
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