| 基体辅助激光解吸电离质谱法对DNA样品的高分辨率测定 |
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| 引用本文： | 邓慧敏,黎军,赖志辉,查庆民,赵善楷.基体辅助激光解吸电离质谱法对DNA样品的高分辨率测定[J].高等学校化学学报,1999,20(6):957-959. |
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| 作者姓名： | 邓慧敏 黎军 赖志辉 查庆民 赵善楷 |
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| 作者单位： | 中山大学测试中心, 广州 510275 |
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| 摘 要： | 基体辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)法因其具有测定质量范围大、灵敏度高、速度快及精确度好等优点,近年来已成为测定多肽、蛋白质、核酸、多糖等生物大分子分子量及其一级结构的有力工具.
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| 关 键 词： | 基体辅助激光解吸电离 DNA 高分辨率质谱图 |
| 收稿时间： | 1999-01-23 |
High Resolution Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis of DNA |
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| DENG Hui-Min,LI Jun,LAI Zhi-Hui,ZHA Qing-Min,ZHAO Shan-Kai.High Resolution Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis of DNA[J].Chemical Research In Chinese Universities,1999,20(6):957-959. |
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| Authors: | DENG Hui-Min LI Jun LAI Zhi-Hui ZHA Qing-Min ZHAO Shan-Kai |
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| Institution: | Instrumental Analysis & Research Center, Zhongshan University, Guangzhou, 510275 |
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| Abstract: | High resolution mass
spectra of DNA samples were acquired by using matrix-assisted laser desorption/ionization
time-of-flight mass spectrometry(MALDI-TOF-byMS) using mixture of 3-hydroxypiclinic acid
and picnilic acid as a matrix. The resolutions of d(C)10, d(G)10,
d(T)18, and d(TTACTCTGTTAATGTCTTTG) mono-isotope peaks have reached 5 513, 3
895, 10 468 and 8 769 respectively. The experiment results showed that the DNA samples
with a higher purity and stability could get higher resolutions. C18 Sep-Pak cartridge, NH+4
cation-exchange resin and nitrocellulose(NC) film substrate have been applied to
eliminating Na+, K+ ions and other impurities from DNA syntheses,
and to purifying the analytes and obtaining high resolution mass spectra with enhanced
sensitivity and reproducibility. |
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| Keywords: | DNA |
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