| 酶催化-荧光猝灭法测定药物中的万古霉素 |
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| 引用本文： | 冯霞光,张敏,赵虎,王怀友.酶催化-荧光猝灭法测定药物中的万古霉素[J].高等学校化学学报,2007,28(7):1270-1272. |
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| 作者姓名： | 冯霞光 张敏 赵虎 王怀友 |
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| 作者单位： | 山东师范大学化学化工与材料科学学院,济南,250014 |
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| 摘 要： | 根据万古霉素对产物的荧光具有猝灭作用的原理, 本文研究了万古霉素的荧光猝灭机理, 建立了测定万古霉素的方法. 该方法具有操作简便、快速及选择性好等优点, 可用于注射液中万古霉素含量的测定, 与药典法对照, 结果令人满意.
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| 关 键 词： | 万古霉素 酪氨酸 辣根过氧化酶 H2O2 荧光猝灭 |
| 文章编号： | 0251-0790（2007）07-1270-03 |
| 收稿时间： | 2006-11-24 |
| 修稿时间： | 2006-11-24 |
Determination of Vancomycin in Injection via Enzyme Catalysis-Fluorescence Quenching Method |
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| FENG Xia-Guang,ZHANG Min,ZHAO Hu,WANG Huai-You.Determination of Vancomycin in Injection via Enzyme Catalysis-Fluorescence Quenching Method[J].Chemical Research In Chinese Universities,2007,28(7):1270-1272. |
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| Authors: | FENG Xia-Guang ZHANG Min ZHAO Hu WANG Huai-You |
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| Institution: | College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China |
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| Abstract: | A new method for determining vancomycin was established based on the principle of fluorescence quenching. The mechanism of the fluorescence quenching was discussed. A linear relationship is obtained between F0/F and the concentration of vancomycin in the range of 40.0—450.0 μg/mL with the correlation coefficiency 0.9991.The detection limit was 10.91 μg/mL and the relative standard derivative was 0.70%. The effects of pH, foreign ions, standing time on the determination of vancomycin were examined. The results obtained by this method were agreed with those by the official method. |
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| Keywords: | Vancomycin Tyrosine Horseradish peroxidase H_2O_2 Fluorescence quenching |
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