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| 吡啶离子液体双水相-高效液相色谱法同时测定牛奶中3种喹诺酮药物残留 | |
| 引用本文： | 曾延波,赵弟海,李蕾,王练,沈兵,奚奇辉,张萌萌.吡啶离子液体双水相-高效液相色谱法同时测定牛奶中3种喹诺酮药物残留[J].高等学校化学学报,2009,30(10):1956-1959. |
| 作者姓名： | 曾延波 赵弟海 李蕾 王练 沈兵 奚奇辉 张萌萌 |
| 作者单位： | 1. 嘉兴学院生物与化学工程学院,嘉兴,314001 2. 嘉兴学院生物与化学工程学院,嘉兴,314001;江西理工大学材料与化学工程学院,赣州,341000 3. 嘉兴出入境检验检疫局,嘉兴,314001 |
| 基金项目： | 浙江省科技计划，浙江省自然科学基金，嘉兴市科技计划 |
| 摘 要： | 建立了吡啶离子液体双水相-高效液相色谱同时测定牛奶中噁喹酸、萘啶酸及氟甲喹3种喹诺酮药物残留的方法. 牛奶样品经氯化钠和磷酸混合溶液提取后, 采用吡啶离子液体N-乙基-2-甲基吡啶溴化盐(EMPy]Br)和K2HPO4形成的双水相体系萃取富集, 以0.1%磷酸水溶液-乙腈为流动相, 梯度洗脱, 紫外检测. 该方法对噁喹酸、萘啶酸和氟甲喹测定的线性范围分别为0.3~15, 0.5~20和0.5~25 μg/mL, 相关系数(r)均大于0.9997, 3种药物的检出限在8~10 μg/kg之间. 对不同加标浓度的牛奶样品测定, 绝对回收率均在86.4%~94.8%范围内, 相对标准偏差为3.6%~8.3%. 该方法对牛奶中喹诺酮药物残留的检测具有简单、快速、环保和灵敏度高等优点. |
| 关 键 词： | 双水相 吡啶离子液体 高效液相色谱 喹诺酮药物检测 牛奶 |
| 收稿时间： | 2009-03-09 |
Simultaneous Determination of Three Quionlones Residues in Milk by Pyridinium Ionic Liquid-based Aqueous Two-phase Systems Coupled with High Performance Liquid Chromatography |
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| ZENG Yan-Bo,ZHAO Di-Hai,LI Lei,WANG Lian,SHEN Bing,XI Qi-Hui,ZHANG Meng-Meng.Simultaneous Determination of Three Quionlones Residues in Milk by Pyridinium Ionic Liquid-based Aqueous Two-phase Systems Coupled with High Performance Liquid Chromatography[J].Chemical Research In Chinese Universities,2009,30(10):1956-1959. | |
| Authors: | ZENG Yan-Bo ZHAO Di-Hai LI Lei WANG Lian SHEN Bing XI Qi-Hui ZHANG Meng-Meng |
| Institution: | ZENG Yan-Bo1,ZHAO Di-Hai1,2,LI Lei1,WANG Lian3,SHEN Bing3,XI Qi-Hui3,ZHANG Meng-Meng3(1.School of Biological and Chemical Engineering,Jiaxing University,Jiaxing 314001,China,2.School of Material and Chemical Engineering,Jiangxi University of Technology,Ganzhou 341000,3.Jiaxing Entry-Exit Inspection and Quarantine Bureau,China) |
| Abstract: | A method was developed for the analysis of three quinolones(oxolinic acid,nalidix acid and flumequine) in milk by pyridinium ionic liquid-based aqueous two-phase systems coupled with high performance liquid chromatography.Milk samples were extracted with the mixed solutions consisting of sodium chloride and phosphoric acid.Then extracts were enriched by aqueous two-phase systems composed of the ionic liquid pyridine N-ethyl-2-methyl-pyridine bromide salt(\Br) and K2HPO4.The separation of the analytes was ac... |
| Keywords: | Aqueous two-phase systems Pyridinium ionic liquid High performance liquid chromatography Determination of quinolone Milk |
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