大鼠肝脏纤维化病变的核磁共振研究

利用高分辨魔角旋转核磁共振技术(HR-MAS NMR)以及主成分分析法(PCA)对大鼠肝脏组织纤维化病变前后以及不同程度病变的代谢产物变化进行了研究. 结果表明, 纤维化病变使得肝脏脂肪含量增加; 葡萄糖、糖原、牛磺酸以及肌醇的含量相对减小. 相对磷酸胆碱(PC)而言, 胆碱(Cho)及甘油磷酸胆碱(GPC)降低. 随着正常至纤维化病变程度的加深, 脂肪含量有一个先显著上升, 再缓慢下降的趋势; 而不同程度纤维化病变阶段之间其它代谢产物的变化不明显. 主成分分析证实了上述的结果, 正常与纤维化病变样本中代谢产物的组成存在较大的差异性, 可被明显分为两组; 而各纤维化病变阶段之间的差异不明显.

1H Nuclear Magnetic Resonance Applied to Hepatic Fibrosis of Rat Liver Tissues

The metabolite profiles from rat hepatic fibrosis were investigated by using high-resolution magic-angle spinning 1H nuclear magnetic resonance(HR-MAS NMR) and principal component analysis(PCA) on the intact tissue. The metabolite characters of hepatic fibrosis were directly observed on 1H NMR spectra by the elevated level of lipids, depleted levels of glucose, glycogen, taurine and inositol, and the decreased levels of choline(Cho) and glycerophosphocholine(GPC) compared with that of phosphocholine(PC). There was a dramatic tendency observed for the level of lipids from the normal to the aggravated stages of hepatic fibrosis, which ascended greatly at first and descended a little bit at the last stage. However, no evident changes of other metabolites in different hepatic fibrosis stages were observed. The results of PCA manifested that hepatic fibrosis tissue samples were separated well from those of the control and no obvious diversities were observed among the samples at different hepatic fibrosis stages.