魔角旋转核磁共振代谢组学方法对镧、铈急性生物效应的比较研究

利用高分辨魔角旋转核磁共振(MAS 1H NMR)技术对腹腔注射不同剂量2, 10, 50 mg/kg(体重)]的硝酸镧La(NO3)3]和硝酸铈Ce(NO3)3] 的雄性Wistar大鼠肝、肾组织的MAS 1H NMR谱进行比较分析, 研究了La(NO3)3和Ce(NO3)3的急性生物效应, 并结合模式识别技术对不同剂量La(NO3)3和Ce(NO3)3的急性生物效应进行了分类. 研究结果表明, La(NO3)3对大鼠的急性毒性主要表现为肝毒, Ce(NO3)3对大鼠肝、肾同时造成损伤. 该方法可用于其它稀土及金属化合物的毒性预测和毒理学研究.

Comparative Investigation on Acute Biological Effect of Lanthanum and Cerium by MAS 1H NMR-based Metabonomic Approach

High-resolution magic-angle-spinning(MAS) ()~1H NMR spectroscopic and pattern recognition(PR) based methods were applied to compare the different acute biochemical effects between La(NO_(3))_(3)- and(Ce(NO_(3))_(3))-treated rats.Male Wistar rats were treated with various doses(2,10,(50 mg/kg) body weight) of La(NO_(3))_(3) and Ce(NO_(3))_(3),and MAS ()~1H NMR spectra of the intact liver and kidney tissues were analyzed by using principal components analysis to extract metabolic information.The target lesion of La(NO_(3))_(3) to liver and Ce(NO_(3))_(3) to both liver and kidney were found by MAS ()~1H NMR-PR methods. This work illustrated that the combination of NMR and pattern recognition technique is a powerful method to study the biochemical effects induced by xenobiotics.