The Effects of Citrato Molybdate on the Growth,Rate of Azotobacter Vinelandii |
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| Authors: | QIU Xue-Hui DONG En-Heng ZHOU Zhao-Hui LONG Ming-Nan ZHANG Feng-Zhang XU Liang-Shu WAN Hui-Lin |
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| Institution: | 1. Department of Biology, Xiamen University, Xiamen 361005;
2. Department of Chemistry, Xiamen University, Xiamen 361005 |
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| Abstract: | An iron-molybdenum cofactor (FeMoco or M-cluster) of nitrogenase is believed to be the active center for the N2-binding and reduction. Recent X-ray crystal structure of MoFe-protein revealed the FeMoco as FeS3Fe3(S)3Fe3S3Mo(R-homocitrate) cluster and the biosynthesis of FeMoco requires at least six nif gene products. FeMoco should be assembled firstly and then inserted into the FeMoco-deficient dinitrogenase. It has been known that the NifB-co, the product of nifB, is an iron and sulfur-containing precursor of FeMoco. However, how the molybdenum and homocitrate are assembled into molybdenum free NifB-co remains unknown. |
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