| AuNPs/Sol-gel复合膜法固定乙酰胆碱酯酶生物传感器检测有机磷农药 |
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| 引用本文： | 孙春燕,李宏坤,平红,王二雷,张民伟,刘静波.AuNPs/Sol-gel复合膜法固定乙酰胆碱酯酶生物传感器检测有机磷农药[J].高等学校化学学报,2011,32(11):2533-2538. |
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| 作者姓名： | 孙春燕 李宏坤 平红 王二雷 张民伟 刘静波 |
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| 作者单位： | 吉林大学军需科技学院,长春,130062 |
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| 基金项目： | 国家自然科学基金(批准号:20905031)资助 |
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| 摘 要： | 基于有机磷农药对乙酰胆碱酯酶(Acetylcholinesterase,AChE)的抑制作用,用金纳米粒子(Au nanoparticles,AuNPs)与壳聚糖/SiO2杂化溶胶-凝胶构成复合固酶基质,将AChE固定于玻碳电极表面,制备了电流型AChE生物传感器选用久效磷进行实验,以氯化硫代乙酰胆碱为底物,建立了电化...
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| 关 键 词： | 乙酰胆碱酯酶 金纳米粒子 溶胶-凝胶 生物传感器 久效磷 |
| 收稿时间： | 2010-11-17 |
Determination of Organophosphorus Pesticides Using a Biosensor Based on Acetylcholinesterase Immobilized by Au Nanoparticles/Sol-gel Composite Membrane |
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| SUN Chun-Yan,LI Hong-Kun,PING Hong,WANG Er-Lei,ZHANG Min-Wei,LIU Jing-Bo.Determination of Organophosphorus Pesticides Using a Biosensor Based on Acetylcholinesterase Immobilized by Au Nanoparticles/Sol-gel Composite Membrane[J].Chemical Research In Chinese Universities,2011,32(11):2533-2538. |
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| Authors: | SUN Chun-Yan LI Hong-Kun PING Hong WANG Er-Lei ZHANG Min-Wei LIU Jing-Bo |
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| Institution: | SUN Chun-Yan,LI Hong-Kun,PING Hong,WANG Er-Lei,ZHANG Min-Wei,LIU Jing-Bo (College of Quartermster Technology,Jilin University,Changchun 130062,China) |
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| Abstract: | Based on the inhibition effects of organophosphorus pesticides on acetylcholinesterase(AChE),an amperomerometric biosensor was developed by immobilizing AChE on glassy carbon electrode with the compo-site film of Au nanoparticals and chitosan-silica hybrid sol-gel.Using acetylthiocholine chloride as the substrate,the electrochemical determination method for organophosphorus pesticide was established with monocrotophos as a representative model for sensitivity tests.The experimental conditions were optimized... |
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| Keywords: | Acetylcholinesterase(AChE) Au nanopaticle Sol-gel Biosensor Monocrotophos |
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