| 基于选择离子集成谱的中药质量评价新方法 |
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| 引用本文： | 张玉峰,范骁辉,瞿海斌.基于选择离子集成谱的中药质量评价新方法[J].高等学校化学学报,2011,32(11):2515-2520. |
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| 作者姓名： | 张玉峰 范骁辉 瞿海斌 |
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| 作者单位： | 浙江大学药物信息学研究所, 杭州 310058 |
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| 基金项目： | 国家重大科技专项“重大新药创制”项目(批准号:2009ZX09313-036,2009ZX09304-002); 国家自然科学基金(批准号:81001687)资助 |
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| 摘 要： | 采用液相色谱-质谱(LC-MS)联用技术建立了不同产地的双丹方(丹参和丹皮)煎剂的指纹图谱, 并提出一种将二维的LC-MS数据转化为一维的选择离子集成谱(ISIC)的方法. 通过主成分分析考察了ISIC与总离子流色谱图(TIC)和保留时间校正后的TIC对不同产地药材的区分能力. 结果表明, ISIC能全面反映不同产地的丹参和丹皮成分间的差异; 而TIC由于色谱峰重叠严重, 对不同产地的药材无区分能力, 保留时间校正后的TIC仅能反映不同产地丹参成分间的差异. 该方法运算速度快, 适于大批量样本分析, 生成的ISIC信息量大, 且不需校正保留时间, 可直接用于后续的统计分析.
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| 关 键 词： | 选择离子集成谱 双丹方 液相色谱-质谱联用技术 |
| 收稿时间： | 2010-12-03 |
Novel Approach for Quality Evaluation of Traditional Chinese Medicine Based on Integrated Selected-ion Chromatograms |
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| ZHANG Yu-Feng,FAN Xiao-Hui,QU Hai-Bin.Novel Approach for Quality Evaluation of Traditional Chinese Medicine Based on Integrated Selected-ion Chromatograms[J].Chemical Research In Chinese Universities,2011,32(11):2515-2520. |
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| Authors: | ZHANG Yu-Feng FAN Xiao-Hui QU Hai-Bin |
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| Institution: | Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China |
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| Abstract: | A liquid chromatography-mass spectrometry(LC-MS) based fingerprinting method was developed to study the Shuangdan extracts prepared by Danshen and Danpi from different habits;the two-way LC-MS datasets were converted to one-way integrated selected-ion chromatograms(ISICs) by our homely-made software and their discriminate power together with total ion chromatograms(TICs) and retention time corrected TICs were evaluated by principle component analysis.The results showed that ISICs surpassed TICs and retentio... |
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| Keywords: | Integrated selected-ion chromatogram(ISIC) Shuangdan formula Liquid chromatography-mass spectrometry(LC-MS) |
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