99mTcN-MIBI在体外肿瘤细胞内的摄取及其与99mTc-MIBI的比较研究

研究了结肠癌细胞CL-187、肺腺癌细胞L-973以及乳腺癌细胞MCF-7分别对^99mTcN-MIBI和^99mTc-MIBI配合物的摄取情况.结果表明,^99mTcN-MIBI在3种肿瘤细胞内培养20～40min时的摄取达到极大值,并且摄取量明显高于^99mTc-MIBI,特别是在结肠癌细胞CL-187中.^99mTcN-MIBI在乳腺癌细胞MCF-7内的摄取量最高,培养40min时的单位细胞摄取百分率约为30%/105个细胞;在结肠癌细胞CL-187内的摄取量次之;在肺腺癌细胞L-973内的摄取量最低.细胞摄取条件实验结果表明:细胞浓度和培养温度的变化均会对细胞摄取产生影响.

Uptake of 99mTcN-MIBI in Tumor Cells in vitro and the Comparison of it with 99mTc-MIBI

In this study, the uptake of ^99mTcN-MIBIand₉₉mTc-MIBIwere investigated by colorectal cancer cell line CL-187, lung cancer cell line L-973 and breast cancer cell line MCF-7 in vitro, respectively.The results of uptake kinetics shows that ^99mTcN-MIBI has maximal accumulations between 20 and 40 min after incubation in all of these tumor cells, and the accumulation(D%) of ^99mTcN-MIBIwas found to be significantly higher than that of ^99mTc-MIBI, especially in colorectal cancer cell line CL-187. Up take of ^99mTcN-MIBIdecreased in the order MCF-7→CL-187→L-973. The maximal accumulation occurring at 40 min after incubation of₉₉mTcN-MIBI in MCF-7 with the uptake rate about 30% per 10⁵cells.The influences of the culture conditions, such as cell concentration and incubation temperature, on the accumulation were also investigated, and the uptake of ^99mTc-complexes by cells were affected significantly by the culture conditions.