| 金表面抗原抗体的固定及其荧光法的测定 |
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| 引用本文： | 姜雄平,许丹科,刘耀清,马立人.金表面抗原抗体的固定及其荧光法的测定[J].高等学校化学学报,2000,21(11):1664-1666. |
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| 作者姓名： | 姜雄平 许丹科 刘耀清 马立人 |
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| 作者单位： | 1. 军事医学科学院放射医学研究所, 北京 100850;
2. 解放军总后勤部卫生部药品仪器检验所, 北京 100071 |
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| 基金项目： | 中国人民解放军“九五”医药卫生科研基金! (批准号 :98Z0 70 )资助 |
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| 摘 要： | 抗原或抗体的固定是制作免疫传感器的基础 .免疫传感器由固定于载体的具有识别作用的抗原或抗体以及指示免疫反应的换能器组成 .金表面自组装技术固定生物分子既可用电化学检测 ,也可用光学检测和质量检测 ,因而近年来用金表面自组装技术固定生物分子的研究越来越多 .其原理是利用金硫键固定含巯基的化合物 ,然后用偶联剂将生物分子连接固定在金表面 ,或者直接将有巯基的生物分子固定在金表面 1,2 ] .本文报道了在金表面自组装技术固定兔 Ig G和山羊抗兔 Ig G的方法基础上 ,建立了抗体竞争法、夹心法和抗体过剩法测定兔 Ig G和山羊抗兔 …
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| 关 键 词： | 免疫传感器 自组装 荧光 |
| 收稿时间： | 2000-01-21 |
Immobilization of Antigen and Antibody on Gold Surface and Determination Using Fluorescent Method |
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| JIANG Xiong-Ping,XU Dan-Ke,LIU Yao-Qing,MA Li-Ren.Immobilization of Antigen and Antibody on Gold Surface and Determination Using Fluorescent Method[J].Chemical Research In Chinese Universities,2000,21(11):1664-1666. |
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| Authors: | JIANG Xiong-Ping XU Dan-Ke LIU Yao-Qing MA Li-Ren |
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| Institution: | 1. Institute of Radiomedicine, Academy of Military Medical Sciences, Beijing 100850, China;
2. Institute for Drug Control of PLA, Beijing 100071, China |
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| Abstract: | A Gold surface was modified with N-acetylcysteine to produce a self-assembled monolayer and then a single layer film of rabbit IgG was immmobilized on modified gold surface covalently using carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS). The competitive method(Ⅰ), the sandwich method(Ⅱ) and the surplus method(Ⅲ) were developed to determine rabbit IgG or goat anti rabbit IgG, respectively. A luminescence analyzer connected with a fluorescence microscope was employed to detect the light intensity of labeled sheep anti rabbit IgG. The results of sensitivity and dynamic range were obtained as 25 mg/L and 25-600 mg/L for method(Ⅰ), 1.0 mg/L and 1-200 mg/L for method(Ⅱ) and 25 mg/L and 25-500 mg/L for method(Ⅲ), respectively. |
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| Keywords: | Immunosensor Self assembled monolayer Fluorescence |
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