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Co@SiO2核壳式纳米磁性粒子的合成、性质表征及在细胞分离和细胞芯片上的应用
引用本文:郭小英,王永宁,顾林岗,贺艳峰,张春秀,唐祖明,陆祖宏.Co@SiO2核壳式纳米磁性粒子的合成、性质表征及在细胞分离和细胞芯片上的应用[J].高等学校化学学报,2006,27(9):1725-1728.
作者姓名:郭小英  王永宁  顾林岗  贺艳峰  张春秀  唐祖明  陆祖宏
作者单位:东南大学生物科学与医学工程系,中国科学院理化技术研究所,江苏省公安厅物证鉴定中心,东南大学公共卫生学院,东南大学生物科学与医学工程系,东南大学生物科学与医学工程系,东南大学生物科学与医学工程系 吴健雄生物分子与电子学国家重点实验室,南京210096,北京100080,南京210024,南京210009,吴健雄生物分子与电子学国家重点实验室,南京210096,吴健雄生物分子与电子学国家重点实验室,南京210096,吴健雄生物分子与电子学国家重点实验室,南京210096
基金项目:国家自然科学基金(批准号:60121101,60223002)和国家高新技术研究发展计划项目(批准号:2002AA222041,2004AA302070)资助.
摘    要:合成了Co@SiO2核壳式纳米粒子,并采用透射电镜(TEM)、X射线衍射(XRD)、扫描电镜(SEM)和振动样品磁强计(VSM)对其形状、尺寸、荧光及磁特性进行了表征,探讨了其在细胞分离和细胞芯片上的应用和原理.

关 键 词:核壳式  纳米磁珠  细胞芯片  细胞分离
文章编号:0251-0790(2006)09-1725-04
收稿时间:09 21 2005 12:00AM
修稿时间:2005-09-21

Synthesis Property Characterization of Core-shell Co@ Si02 Magnetic Nanoparticle and Its Biomedical Applications to Cell Separation and Cell Chip
GUO Xiao-Ying , WANG Yong-Ning, GU Lin-Gang, HE Yan-Feng, ZHANG Chun-Xiu , TANG Zu-Ming , LU Zu-Hong.Synthesis Property Characterization of Core-shell Co@ Si02 Magnetic Nanoparticle and Its Biomedical Applications to Cell Separation and Cell Chip[J].Chemical Research In Chinese Universities,2006,27(9):1725-1728.
Authors:GUO Xiao-Ying  WANG Yong-Ning  GU Lin-Gang  HE Yan-Feng  ZHANG Chun-Xiu  TANG Zu-Ming  LU Zu-Hong
Institution:1. State Key Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096, China; 2. Technical Institute of Physical and Chemistry, Chinese Academy of Sciences, Beijing 100080, China; 3. Division of the Criminal Investigation, Department of Jiangsu Provincial Public Security, Nanjing 210024, China; 4. College of Public Health, Southeast University, Nanjing 210009, China
Abstract:In this paper, Co@SiO_ 2 nanoparticles with the apparent core-shell magnetic nanostructure were synthesized by reducing and hydrolysis in aqueous solutions. The TEM, VSM, SEM, and the confocal fluorescence microscope were utilized to characterize the morphology, size, magnetism of the Co@SiO_ 2 nanoparticles, and the core-shell materials were applied to the cell separation, immunophenotyping of leukemias and lymphomas diagnostics chip, in which strenuous procedures were omitted by the use of the magnetic core-shell nanoparticles, and separation. These core-shell nanoparticles were proved to have obvious cores and shell, and the cores have strong magnetism, and the shell protected these nanosized metal particles from coagulation due to anistropic dipolar attraction and oxidation very well. However this method could not limited the original cores below the size of 10 nm, which is the critical size of superparamagnetisizm, as a result coagulation due to remanent magnetism limited this core-shell particles from further applications in bioseparation.
Keywords:Core-shell  Magnetic nanoparticle  Immunophenotyping of leukemias and lymphomas  Cell separation  
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