|
|||||
|
|
| 给药硝酸钕后大鼠完整肝组织及肝组织提取物的NMR代谢组学研究 | |
| 引用本文： | 廖沛球,魏来,刘威,吴亦洁,李晓晶,倪嘉缵,裴奉奎.给药硝酸钕后大鼠完整肝组织及肝组织提取物的NMR代谢组学研究[J].高等学校化学学报,2009,30(6). |
| 作者姓名： | 廖沛球 魏来 刘威 吴亦洁 李晓晶 倪嘉缵 裴奉奎 |
| 作者单位： | 1. 中国科学院长春应用化学研究所,长春130022;中国科学院研究生院,北京100049 2. 吉林省中医药科学院,长春,1300121 3. 中国科学院长春应用化学研究所,长春,130022 |
| 摘 要： | 通过分析不同给药剂量硝酸钕Nd（NO3）3]2，10，50mg／kg（体重）]后，雄性Wistar大鼠完整肝组织的MAS1HNMR谱和肝组织提取物的^1H NMR谱，结合肝组织病理切片图，研究了稀土化合物Nd（NO3）3在大鼠体内的急性生物效应．利用模式识别方法对给药Nd（NO3）3组和对照组大鼠肝组织^1H NMR谱图数据进行了分析．结果表明，腹腔注射Nd（NO3）3后，大鼠肝脏中甘油三酯、亮氨酸（异亮氨酸）、乳酸、丙氨酸、丙酮酸、磷酸胆碱和葡萄糖含量升高，氮氧三甲胺含量降低．肝脏病理图显示，50mg／kg（体重）组大鼠肝细胞可见微小坏死灶和门管区炎细胞轻度增多．推测硝酸钕能影响大鼠肝脏中能量代谢（糖代谢和脂肪代谢）和氨基酸代谢，对大鼠肝脏造成损伤，且其损伤程度随剂量的增加有增强趋势． |
| 关 键 词： | 核磁共振 代谢组学 模式识别 肝 硝酸钕 生物效应 |
Metabonomic Studies on Intact Livers and Liver Extracts from Rats Treated with Neodymium Nitrate |
|
| LIAO Pei-Qiu,WEI Lai,LIU Wei,WU Yi-Jie,LI Xiao-Jing,NI Jia-Zuan,PEI Feng-Kui.Metabonomic Studies on Intact Livers and Liver Extracts from Rats Treated with Neodymium Nitrate[J].Chemical Research In Chinese Universities,2009,30(6). | |
| Authors: | LIAO Pei-Qiu WEI Lai LIU Wei WU Yi-Jie LI Xiao-Jing NI Jia-Zuan PEI Feng-Kui |
| Institution: | LIAO Pei-Qiu1,2,WEI Lai1,LIU Wei3,WU Yi-Jie1,LI Xiao-Jing1,NI Jia-Zuan1,PEI Feng-Kui1(1.Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China,2.Graduate School of the Chinese Academy of Sciences,Beijing 100049,3.Jilin Provincial Academy of Traditional Chinese Medicine,Changchun 130021,China) |
| Abstract: | The biochemical effects of neodymium nitrate were studied by NMR spectroscopic-based metabonomic approach.Male Wistar rats were treated intraperitoneally with different doses(2,10 and 50 mg/kg body weight) of Nd(NO3)3.Liver samples from Nd3+-treated rats were analyzed either by 600 MHz 1H MAS NMR techniques for intact liver or using high resolution(liquid state) 1H NMR for liver chloroform/water/methanol extracts.All NMR spectra data were analyzed by pattern recognition using principal components analysis.L... |
| Keywords: | Nuclear magnetic resonance Metabonomics Pattern recognition Liver Neodymium nitrate Biochemical effect |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |