| 脂肪酶在负离子化PET表面的单分子层自组装膜研究 |
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| 引用本文： | 林贤福,何琳,徐立恒,吕德水,陈志春.脂肪酶在负离子化PET表面的单分子层自组装膜研究[J].高等学校化学学报,2001,22(4):683-686. |
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| 作者姓名： | 林贤福 何琳 徐立恒 吕德水 陈志春 |
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| 作者单位： | 浙江大学分析测试中心, |
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| 摘 要： | 研究了脂肪酶分子在表面负离子化聚对苯二甲酸乙二醇酯（PET）薄膜表面的分子自组装，由AFM和FTIR－ATR等方法研究脂肪酶单分子层膜的表面结构；活力测定表明：脂肪酶／PET自组装单分子层膜的酶活力表现率约为2.0左右。
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| 关 键 词： | 脂肪酶 分子自组装 表面负离子化聚对苯一甲酸乙二醇酯薄膜 单分子层膜 酶活力 |
| 文章编号： | 0251-0790（2001）04-0683-04 |
Studies on the Lipase Monolayer Film Self-assembly on the Nagatively Charged Poly(ethylene terephthalate) Substrate |
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| LIN Xian-Fu,HE Lin,XU Li-Heng,Lü De-Shui,CHEN Zhi-Chun.Studies on the Lipase Monolayer Film Self-assembly on the Nagatively Charged Poly(ethylene terephthalate) Substrate[J].Chemical Research In Chinese Universities,2001,22(4):683-686. |
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| Authors: | LIN Xian-Fu HE Lin XU Li-Heng Lü De-Shui CHEN Zhi-Chun |
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| Abstract: | The PET CO - 2 film was prepared and the lipase was assembled on the surface of the PET CO - 2 substrate. The structure of the surface and activity of lipase/PET monolayer were studied by FTIR ATR and AFM and other methods. As shown in the results, AFM images of lipase/PET monolayer indicated that the surface fluctuation is in the range of nanometer scale and the depth of monolayer is less than 15 nanometers. The performance activity of lipase/PET monolayer self assembly film is above 2.0. |
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| Keywords: | Lipase monolayer Molecular self assembly Poly(ethylene terephthalate) AFM FTIR ATR |
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