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| 锰(Ⅲ)Corrole配合物催化DNA氧化断裂 | |
| 引用本文： | 刘海洋,刘兰英,张雷,应晓,王湘利,江焕峰,张启光.锰(Ⅲ)Corrole配合物催化DNA氧化断裂[J].高等学校化学学报,2007,28(9):1628-1630. |
| 作者姓名： | 刘海洋 刘兰英 张雷 应晓 王湘利 江焕峰 张启光 |
| 作者单位： | 1. 华南理工大学化学系,广州,510641;香港科技大学化学系,香港 2. 华南理工大学化学系,广州,510641 3. 华南理工大学制药工程系,广州,510641 4. 华南理工大学应用物理系,广州,510641 5. 香港科技大学化学系,香港 |
| 基金项目： | 国家自然科学基金 , 教育部留学回国人员科研启动基金 |
| 摘 要： | 报道了这类单羟基Corrole锰(Ⅲ)配合物对DNA的催化氧化断裂作用. |
| 关 键 词： | 锰 Corrole DNA 氧化断裂 |
| 文章编号： | 0251-0790（2007）09-1628-03 |
| 收稿时间： | 2007-05-08 |
| 修稿时间： | 2007-05-08 |
Oxidative DNA Cleavage Catalyzed by Mn(Ⅲ) Corroles |
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| LIU Hai-Yang,LIU Lan-Ying,ZHANG Lei,YING Xiao,WANG Xiang-Li,JIANG Huan-Feng,CHANG Chi-Kwong.Oxidative DNA Cleavage Catalyzed by Mn(Ⅲ) Corroles[J].Chemical Research In Chinese Universities,2007,28(9):1628-1630. | |
| Authors: | LIU Hai-Yang LIU Lan-Ying ZHANG Lei YING Xiao WANG Xiang-Li JIANG Huan-Feng CHANG Chi-Kwong |
| Institution: | 1. Department of Chemistry, South China University of Technology, Guangzou 510641, China ; 2. Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China; 3. Department of Pharmaceutical Engineering, 4. Department of Applied Physics, South China University of Technology, Guangzou 510641, China |
| Abstract: | Several Mn(Ⅲ) 10-(hydroxylphenyl)-5,15-bis(pentafluoro-phenyl)corroles with the hydroxyl at ortho-, meta- and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the presence of H2O2. The catalytic system was composed of 10 μL 0.5 mg/mL pC DNA 6 in TE buffer solution(pH=8.0, 4.0×10-2 mol/L Tris, 1.0×10-3 mol/L EDTA), 10 μL 1.0×10-5 mol/L Mn(Ⅲ) corrole in DMSO and 10 μL 0.3% H2O2. After 8 h incubation at room temperature, significant oxidative DNA damage could be observed with the ortho-hydroxyl Mn(Ⅲ) corrole showing a better activity as detected by agarose-gel electrophoresis. Plasmid DNA was completely damaged after 12 h incubation. These observations show Mn(Ⅲ) corrole can be used as an artificial model of nuclease. |
| Keywords: | Manganese Corrole DNA Oxidative cleavage |
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