基于宿主细胞中磷脂“自然嵌合”的杆状病毒叶酸修饰

正杆状病毒是节肢动物特异性的DNA病毒,具有载体容量大、增殖滴度高、易于大量扩增且对脊椎动物无致病性等优点,在新型基因载体及疫苗开发等方面具有广阔的应用前景1,2].然而,杆状病毒缺乏对脊椎动物细胞的靶向性,因此有必要对杆状病毒进行修饰3~5].叶酸(FA)是一种人体必需的维生素,具有分子量小、稳定性好、无免疫原性及易于与其它载体或药物键合等优点.由于叶酸受体在多种肿瘤细胞中过度表达,叶酸已成为一种常见的通用型肿瘤靶向分子6~9].本文采用宿主细胞中磷脂"自

Modification of Baculovirus with Folic Acid based on Phospholipids Self-insertion in Host Cells?

Baculovirus has the advantages such as outstanding biosafety, large cloning capacity and non-repli-cation in the transduced cells. However, their in vivo applications are hampered for lacking of specificity. Therefore, modification of baculovirus with targeting molecules is urgently needed. Herein, a method is pro-posed for the modification of folic acid(FA) on baculovirus based on phospho-lipids self-insertionin host cells. Immunofluorescent colocalization and transmission electron microscope(TEM) results showed that the modified baculovirus kept their integrity while maintaining their infectivity. Thus the method offers opportunities for the development of tumor-targeting gene vectors and vaccines.