1-乙酰基-3-(2-羟基-4,6-二甲氧基苯基)-5-苯基-2-吡唑啉的合成及锌离子探针的研究

设计合成了1-乙酰基-3-(2-羟基-4,6-二甲氧基苯基)-5-苯基-2-吡唑啉(4), 测试了其紫外光谱和荧光光谱, 研究了其对锌离子的选择性识别作用. 结果表明, 化合物4作为锌离子荧光探针, 受常见离子的干扰较小, 对于锌离子有着较高的选择性和较低的检出限.

Synthesis of 1-Acetyl-3-(2-hydroxyl-4,6-dimethoxylphenyl)-5-phenyl-2-pyrazoline and Studies on Its Zinc Ion Probe

The development of Zn2+ ion chemosensors has received considerable attention. The use of Zn2+-specific chemosensors in biological systems promises to close the knowledge gap between the well-defined structural biochemistry of zinc and the understanding of zinc homeostasis and action. The synthesis of 1-acetyl-3-(2-hydroxy-4,6-dimethoxy-phenyl)-5-phenyl-2-pyrazoline(4) and the studies of it as fluorescent sensors for Zn2+ ion were reported. Its UV-Vis and fluorescence spectrum were detected. The influences of 14 metal ions such as Cu2+, Zn2+, Co2+ etc. on their fluorescence properties were measured. Spectroscopic studies revealed that the compound 4 had rather strong affinity toward zinc ion. The specificity of the probe 4 toward Zn2+ was determined. Nearly no fluorescence intensity changes were observed in emission spectra with Cu2+, Co2+, Ni2+, Na+, K+, Ca2+, Cr3+, Cd2+, Al3+, Mn2+, Pb2+, Sr2+ and Sn2+. However, under identical conditions, fluorescence intensity enhanced significantly in the presence of Zn2+. So we have developed a new fluorescent sensor(compound 4). It show high sensitivity and selectivity toward Zn2+ in DMSO aqueous solution.