聚吡咯电化学固定化胆固醇氧化酶电极的电流响应及应用

用电极法测定总胆固醇量,可以通过制备胆固醇氧化酶和胆固醇脂酶的酶膜与氧电极偶联制成传感器或制备以过氧化氢检出方式的传感器来完成,本文研制了一种新型的酶电极,它不需选择电荷传递中间体代替氧分子的作用,本方法利用吡咯单体在水溶液中能电氧化聚合,并伴有对阴离子嵌入聚合物骨架的性质,使酶分子在聚合过程中作为阴离子而嵌

Application and Current Response of Electrochemical and Immobile Polypyrrole Cholesterol Oxidase Electrode

This article reports a new kind of PP/GOX/cholesterol oxidasemodifidd electrode. In the presence of enzyme, pyrrole was electrolysed and precipitated, its monomer was electrochemically oxidized and polymerized to form membrane which carries positive charge. In the process of membrane formation, the anion entered the membrane. After studying the electropolymerizing conditions, the electrochemical behaviour and other properties of this electrode, we found that in order to improve measuring sensitivity and accuration and reduce working potential and diminish background current, Mo6+ should be added to the solution, the catalyst I- is oxidized by H2O2 to form I2, then, the existence of cholesterol can be determined by measuring the reduced current of I2.This method can be applied to measuring tolly cholesteol and the result is satisfactory. Manufacturing this kind of electrodes is significant for modified electrode miniaturization and living body analysis.