首页 | 本学科首页   官方微博 | 高级检索  
     检索      

在不同激发波长下的单壁碳纳米管的拉曼光谱研究
引用本文:滕利荣,初宇卓,张晓萍,王静,韩松,于笑坤,刘兰英.在不同激发波长下的单壁碳纳米管的拉曼光谱研究[J].高等学校化学学报,2005,26(9):1662-1664.
作者姓名:滕利荣  初宇卓  张晓萍  王静  韩松  于笑坤  刘兰英
作者单位:吉林大学生命科学学院,长春130023
基金项目:吉林大学学生科技创新基金
摘    要:采用直流电弧法制备单壁碳纳米管样品,用457.5和632.8nm两种不同的激发光分别测得单壁碳纳米管的正常拉曼光谱和共振拉曼光谱.通过理论分析得到了单壁碳纳米管的直径分布,进一步推测了其类型及结构参数;对单壁碳纳米管的正切拉伸模的成分进行了归属.在632.8nm激发波长下得到了IG/ID值随激光功率变化的曲线,认为在2.5mW时,单壁碳纳米管缺陷的结构可能发生了改变.在用457.5nm波长激发的单壁碳纳米管的拉曼光谱中,首次发现了1421cm-1的拉曼谱峰.

关 键 词:单壁碳纳米管  拉曼光谱  共振  一维电子态密度
文章编号:0251-0790(2005)09-1662-03
收稿时间:09 13 2004 12:00AM
修稿时间:2004-09-13

Studies on Tryptophan Residue Modification and Fluorescence Spectrum of Hyaluronidase
TENG Li-Rong,CHU Yu-Zhuo,ZHANG Xiao-Ping,WANG Jing,HAN Song,YU Xiao-Kun,LIU Lan-Ying.Studies on Tryptophan Residue Modification and Fluorescence Spectrum of Hyaluronidase[J].Chemical Research In Chinese Universities,2005,26(9):1662-1664.
Authors:TENG Li-Rong  CHU Yu-Zhuo  ZHANG Xiao-Ping  WANG Jing  HAN Song  YU Xiao-Kun  LIU Lan-Ying
Institution:1. School of Physics and Information Optoelectronics, Henan University, Kaifeng 475001, China;
2. College of Physics Science, Naikai University, Tianjing 300071, China
Abstract:Tryptophan residues in Hyaluronidase(HAase) were modified by N-bromosuccinimide(NBS). The results indicated that there were eleven tryptophan residues in HAase and one of them was exposed, which was proved to be essential for the activity of the enzyme. The study on fluorescence quenching of HAase showed that KI could not quench all of the fluorescence from Trp residues in HAase . Acrylamide(Acr), a polarized quencher without electronic charge, could quench almost all of the fluorescence from Trp residues in HAase . The collisional quenching constants(K-D) of HAase at different concentrations of Acr were calculated in terms of Stern-Volmer equation. The results implied that some of Trp residues were buried in the interior of HAase, and the Trp residue on the surface of HAase was not located in the hydrophobic pocket.
Keywords:Hyaluronidase(HAase)  Tryptophan(Trp residue)  Chemical modification  Fluorescence quenching
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《高等学校化学学报》浏览原始摘要信息
点击此处可从《高等学校化学学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号