表面等离子共振技术测定生长抑素含量

应用表面等离子共振(SPR)技术建立了一种快速检测生长抑素(SST)含量的方法,在pH=5.0,SST浓度为75μg/mL的最佳偶联条件下,SST在CM5芯片上的偶联值为1231.7Responseunit(RU).选择117.7nmol/L作为固定的大分子量抗体浓度,用抗原-抗体-抗原结合法检测SST纯品,在20～2000pg范围内,SST纯品含量和RU值之间可建立直线相关的标准曲线,R=-0.824,p0.05,变异系数CV=1.3%.所建立的方法测定SST含量的范围为20～2000pg,重复性好且可随时检测,有望用于SST的临床即时检测.

Quantification of Somatostatin with a Surface Plasmon Resonance-based Biosensor

A quick technique of quantifying somatostatin(SST) with surface plasmon resonance(SPR) on the principle of antigen-antibody-antigen assay was established. The optimal coupling of CM5 sensor chip for SST was indicated as 1231.4 response unit(RU) at pH=5.0, and the SST concentration of 75 μg/mL. A negative linear relationship between SST content(20—2000 pg) and RU was obtained using the high molecular mass antibody for SST at 117.7 nmol/L, R=-0.824, p<0.05, coefficient of variation 1.3%. This method can be used to detect SST content at a range from 20 to 2000 pg with good repetition and real-time, which would be useful for clinical diagnosis and intensive care of critically ill patients.