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压电胰岛素-C肽微阵列免疫传感器研究
引用本文:张波,府伟灵,毛琼国,张雪,汤万里,俞凡.压电胰岛素-C肽微阵列免疫传感器研究[J].高等学校化学学报,2004,25(1):44-49.
作者姓名:张波  府伟灵  毛琼国  张雪  汤万里  俞凡
作者单位:1. 第三军医大学西南医院检验科,重庆,400038
2. 中国嘉陵集团生物工程办公室,重庆,400032
3. 信息产业部电子第26研究所,重庆,400060
基金项目:国家科技部“九五”科学仪器攻关项目(批准号:96-A23-04-04),国家高技术计划生物技术领域青年基金,国家自然科学基金(批准号:39870831),解放军“九五”,“十五”课题(批准号:98M064,01MA180,01LO065)资助
摘    要:以AT切型、基频10MHz的镀金膜石英晶体作为换能器,将抗人胰岛素和C肽单克隆抗体固定在石英晶体电极表面,用2×5检测池固定夹具构建一种新型压电胰岛素-C肽微阵列免疫传感器.研究了抗体固定方法、抗体工作浓度、固定量、一致性以及传感器的响应参数如检测温度、时间和特异性等的影响.该微阵列传感器在胰岛素浓度为2.5~160.0mIU/L、C肽浓度为0.375~12.0ng/mL范围内响应特性良好,压电晶体频率偏移值与胰岛素和C肽浓度呈良好的线性关系.将此微阵列传感器用于人血清标本的测定,结果与放射免疫法符合(r为0.92和0.94).此微阵列传感器具有灵敏度高、特异性好,低密度阵列结构,检测通量较高,不需标记,操作简单、能实时在线检测和重复使用等优点,能用于临床实验诊断,具有临床推广应用价值.

关 键 词:压电免疫传感器  微阵列  胰岛素  C肽
文章编号:0251-0790(2004)01-0044-06
收稿时间:2002-09-06

Studies on Piezoelectric Insulin-C Peptide Micro-array Immunosensor
ZHANG Bo,FU Wei-Ling ,MAO Qiong-Guo,ZHANG Xue,TANG Wan-Li,YU Fan.Studies on Piezoelectric Insulin-C Peptide Micro-array Immunosensor[J].Chemical Research In Chinese Universities,2004,25(1):44-49.
Authors:ZHANG Bo  FU Wei-Ling  MAO Qiong-Guo  ZHANG Xue  TANG Wan-Li  YU Fan
Institution:ZHANG Bo1,FU Wei-Ling 1*,MAO Qiong-Guo1,ZHANG Xue1,TANG Wan-Li2,YU Fan3
Abstract:A new 2×5 type of piezoelectric insulin-C peptide micro-array immunosensor was developed for the determination of insulin and C peptide. The energy converters were 10 MHz AT-cut quartz crystals with gold-coated electrodes. The monoclonal antibodies of insulin and C peptide were immobilized onto the surfaces of crystals gold electrodes respectively, then piezoelectric insulin-C peptide micro-array immunosensor was constructed by 2×5 type of immobilization clamping apparatus. The effects of methods, work concentrations, the amount and coherence of immobilization of antibody and the response parameters of the micro-array immunosensor such as temperature, time-cost and specificity etc. were investigated. Under the optimized conditions, the experimental results showed piezoelectric immunosensor had good response to insulin and C peptide whose frequency shifts were linearly dependent on insulin concentration in the range of 2.5—160.0 mIU/L and C peptide concentration in the range of 0.375—12.0 ng/mL. The piezoelectric micro-array immunosensor were used to detect insulin and C peptide in serum, the analytical results given by this approach were in satisfactory agreement with those given by radiometric immunoassay(P>0 05), with correlation coefficients 0 92 and 0 94. The piezoelectric micro-array immunosensor for the determination of insulin and C peptide is of high sensitivity, high specificity, low density micro-array structure, high analysis speed, unnecessary labelling, simple operation, real-time detection and repeated use, etc.. It is suitable for detecting insulin and C peptide of serum in clinical laboratory.
Keywords:Piezoelectric immunosensor  Micro-array  Insulin  C peptide  
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