| 碱性溶液中甲醇分子诱导的肌血球素构象变化 |
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| 引用本文： | 姜丹,储艳秋,陈南生,丁传凡.碱性溶液中甲醇分子诱导的肌血球素构象变化[J].高等学校化学学报,2009,30(11):2139-2145. |
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| 作者姓名： | 姜丹 储艳秋 陈南生 丁传凡 |
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| 作者单位： | 1. 复旦大学化学系激光化学研究所, 上海市分子催化和功能材料重点实验室, 上海 200433;
2. Department of Molecular Biology and Biochemistry, Simon Fraser University, Canada |
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| 摘 要： | 考察了不同pH值的碱性溶液中甲醇分子对诱导肌血球素构象变化的影响. 电喷雾电离质谱(ESI-MS)测量结果表明, 当pH分别为11.0, 11.4, 12.0时, 不同体积分数的甲醇(30%, 50%, 70%及90%)和水混合溶液将诱导肌血球素的构象变化. 当pH=11.0时, 肌血球素在溶液中均以hMb为主要形态存在(即血红素稳定存在于肌红蛋白中); 当pH=11.4时, 30%甲醇中的肌血球素仍以hMb为主, 但升高甲醇比例会导致hMb上的辅基脱落生成aMb(蛋白质分子); 当pH=12.0时, 溶液中的肌血球素均以aMb为主. 但当应用负离子质谱测量模式时, 检测出的肌血球素负离子均以aMb构象为主. 同时采用圆二色谱(CD)及紫外-可见(UV-Vis)光谱等研究发现, pH值和甲醇含量分别决定蛋白质分子的二级和三级结构的变化.
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| 关 键 词： | 蛋白质 分子构象 电喷雾质谱 肌血球素 碱性溶液 |
| 收稿时间： | 2009-07-07 |
Methanol-induced Conformation Change of Myoglobin in Basic Solution |
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| JIANG Dan,CHU Yan-Qiu,CHEN Nan-Sheng,DING Chuan-Fan.Methanol-induced Conformation Change of Myoglobin in Basic Solution[J].Chemical Research In Chinese Universities,2009,30(11):2139-2145. |
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| Authors: | JIANG Dan CHU Yan-Qiu CHEN Nan-Sheng DING Chuan-Fan |
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| Institution: | 1. Shanghai Key Laboratory of Molecular Catalysis and Innovative materials, Laser Chemistry Institute, Department of Chemistry, Fudan University, Shanghai 200433, China;
2. Department of Molecular Biology and Biochemistry, Simon Fraser University, Canada |
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| Abstract: | This paper reports the methanol-induced conformation change of myoglobin in basic solution.According to the results of mass spectrometry,we found that the conformation of myoglobin(Mb) changed in different volume fractions of methanol at pH=11.0,11.4,12.0.And both of the methanol concentration and pH value have some effect.Mb exists as hMb at pH=11.0,aMb at pH=12.0,aMb at pH=11.4 except in 30% volume fraction of methanol solution.Using negative ion mass spectrometry mode,Mb all show mainly as aMb.More infor... |
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| Keywords: | Protein Molecular conformation Electrospray ionization-mass spectrometry Myoglobin Basic solution |
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