有机相中脂肪酶催化糖酯合成的研究

研究了有机相中脂肪酶催化以单糖和乙酸乙烯酯或乙酸酐为底物的糖脂合成反应。建立了定性、定量检测糖酯的方法，考察了７种脂肪酶催化糖酯合成的活力，发现来自假单孢菌属的ＰＳＬ１的活力最高。研究了ＰＳＬ１对不同单糖底物的选择性，发现对甘露醇的选择性最好，转化率可达９５％。研究了反应体系中的含水量对糖酯合成的影响，探讨了酶浓度和温度对反应的影响。

Studies on Synthesis of Monosaccharide Esters Catalyzed by Lipases in Organic Solvents

The synthesis of monosaccharide ester catalyzed by lipase in organic solvents was studied,where monosaccharides and vinyl acetate or acetic anhydride serve as substrates.Quantitative and qualitative methods for determination of monosacchride ester were developed.The activities of lipases from different sources catalyzing synthesis of monosacchride ester were compared and lipase from Pseudmonus S.P.Lipase(PSL1) was the best.We also studied the selectivity of PSL1 to different monosaccharide substrates and found mannitol is the most efficiency substrate to PSL1 and the conversion can reach 95%.The effects of water content in the system,temperature and lipase concentration on the reaction were studied.