Cr3+比色荧光探针的合成及细胞成像应用

以2,3,3-三甲基-3H-苯并e]吲哚和对二甲氨基苯甲醛为原料, 乙醇作溶剂, 在酸性催化条件下, 通过一步反应合成了比色荧光探针B. 在EtOH/HEPES(pH=7.4)体积比为9∶1的混合体系中, 向探针B溶液中加入Cr ³⁺后, 溶液颜色由淡黄色变为紫红色, 说明探针B可以对Cr ³⁺进行裸眼识别. 紫外-可见吸收光谱和荧光发射光谱分析表明, 探针B对Cr ³⁺的选择性好、 灵敏度高且对EDTA有良好的接力识别. 探针B对Cr ³⁺的结合常数K_a=0.28×10 ² mol/L, 检出限为1.90×10 ^-8 mol/L, 该检出限低于世界卫生组织(WHO)规定的饮用水中Cr ³⁺的最大含量(9.60×10 ^-8 mol/L). 利用荧光发射光谱对实际水样中Cr ³⁺的浓度进行了定量检测. 探针B也可应用于对活细胞中Cr ³⁺的检测, 具有较好的应用前景和实用价值.

Synthesis of a Colorimetric Fluorescent Probe of Cr 3+ and Its Application in Cell Imaging ?

Colorimetric fluorescence probe B was synthesized from 2,3,3-trimethyl-3H-benzoe]indole and p-dimethylaminobenzaldehyde using acidic solvent as catalyst and ethanol as solvent. In the mixed system(pH=7.4) of V(EtOH)∶V(HEPES)=9∶1, the color of solution B changed from pale yellow to purple-red after added Cr ³⁺ to probe B solution, indicating that probe B can recognize Cr ³⁺ with naked-eye. Fluorescence emission spectra shows that probe B had good selectivity of Cr ³⁺, high sensitivity and good recognition ability with EDTA. The binding constant(K_a) of probe B on Cr ³⁺ was 0.28×10 ² mol/L and the detection limit was 1.90×10 ^-8 mol/L. The detection limit was lower than the maximum Cr ³⁺ content(9.60×10 ^-8 mol/L) in drinking water stipulated by WHO. In addition, the concentration of Cr ³⁺in actual water samples was quantitatively detected by fluorescence emission spectra. Probe B has been applied to the detection of Cr ³⁺ in living cells, which has a good application prospect and practical value.