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| 改性纳米羟基磷灰石/PLGA复合材料的制备及生物活性 | |
| 引用本文： | 于婷,刘娅,王宇,景遐斌,章培标,陈学思.改性纳米羟基磷灰石/PLGA复合材料的制备及生物活性[J].高等学校化学学报,2009,30(7). |
| 作者姓名： | 于婷 刘娅 王宇 景遐斌 章培标 陈学思 |
| 作者单位： | 1. 中国科学院长春应用化学研究所,高分子物理与化学国家重点实验室,长春,130022;吉林大学公共卫生学院,长春,130021 2. 吉林大学公共卫生学院,长春,130021 3. 中国科学院长春应用化学研究所,高分子物理与化学国家重点实验室,长春,130022 |
| 基金项目： | 国家自然科学基金，国家"八六三"计划 |
| 摘 要： | 以低聚乳酸接枝改性的羟基磷灰石纳米粒子(op-HA)和聚丙交酯-乙交酯(PLGA)制备的生物可降解纳米复合材料(op-HA/PLGA)为研究对象, 采用FTIR, TGA, ESEM和EDX分析其接枝反应、接枝率、表面形貌和钙磷沉积情况, 通过在材料膜表面接种兔成骨细胞进行体外培养, 采用荧光染色、 NIH Image J图像分析和Real-time PCR综合评价细胞在材料表面的形态、黏附面积比、增殖能力和基因表达水平, 以此评价新型骨修复纳米复合材料op-HA/PLGA的表面性质和生物活性. 研究结果表明, op-HA的表面接枝率为8.3%, 掺入至PLGA后可形成富含钙磷的粗糙表面, 促进成骨细胞的黏附、扩展和增殖, 提高Ⅰ型胶原蛋白(Collagen-Ⅰ)、骨形态蛋白-2(BMP-2)和骨连接蛋白(Osteonectin)的基因表达水平, 提高材料的钙磷沉积能力. op-HA/PLGA具有良好的细胞相容性和成骨活性. |
| 关 键 词： | 羟基磷灰石(HA) 聚丙交酯-乙交酯(PLGA) 表面改性 纳米复合材料 成骨活性 |
Preparation and Bioactivity of the Composite of PLGA and Hydroxyapatite Nanocrystals Surface-grafted with L-lactic Acid Oligomer |
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| YU Ting,LIU Ya,WANG Yu,JING Xia-Bin,ZHANG Pei-Biao,CHEN Xue-Si.Preparation and Bioactivity of the Composite of PLGA and Hydroxyapatite Nanocrystals Surface-grafted with L-lactic Acid Oligomer[J].Chemical Research In Chinese Universities,2009,30(7). | |
| Authors: | YU Ting LIU Ya WANG Yu JING Xia-Bin ZHANG Pei-Biao CHEN Xue-Si |
| Institution: | 1. State Key Laboratory of Polymer Physics and Chemistry;Changchun Institute of Applied Chemistry;Chinese Academy of Sciences;Changchun 130022;China;2. School of Public Health;Jilin University;Changchun 130021;China |
| Abstract: | The hydroxyapatite(HA) nanocrystals of 100200 nm in length and 2030 nm in width were hydrothermally synthesized by the reaction of phosphoric acid and calcium hydroxide. Lactic acid oligomer surface grafted HA(op-HA) nanoparticles were obtained by oligomeric lactic acid with a certain molecular weight grafting onto the HA surface to form a Ca carboxylate bond in the absence of any catalyst. The op-HA was further blended with poly(lactide-co-glycolide)(PLGA) to prepare the nanocomposite of op-HA/PLGA. FTIR, ... |
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