分子烙印聚合物固定相分离咖啡因和茶碱的研究

分子烙印是一种新兴的分子识别技术，利用该技术可制备对烙印分子具有“预定”识别能力的高分子聚合物，即分子烙印聚合物（MIP），从而可以对性质和组成相近的组如对映体等进行分离^1,2]，咖啡因与茶碱的分子烙印聚合物的制备以及二者分析已有报道^3-9]，但存在两种完全相反的结论。一种观点认为，即使以咖啡因为烙印分子，所制备的聚合物对咖啡因分子的选择性吸附能力也小于茶碱^3-6]。而另一种观点则认为，在一定条件下，如以咖啡因分子为烙印分子的烙印聚合物对咖啡因分子具有更强的吸附能力^7-9]。本文分别采用茶碱和咖啡因作为烙印分子，以甲基丙烯酸等为功能单体，在不同条件下制备了多种非共价型分子烙印聚合物，并系统地考察了其作 为HPLC固定相对咖啡因和茶碱的分离能力，同时也对烙印分子应具备的条件加以探讨。

Investigation of Separation of Caffeine and Theophylline on Molecular Imprinted Polymer Stationary Phases by High Performance Liquid Chromatography

Molecularly imprinted polymer(MIP) stationary phases against caffeine and against theophylline were prepared. Separation abilities of the phases were investigated by HPLC. The results showed that the theophylline based MIP stationary phase is highly selective to theophylline, and base lined separation of caffeine and theophylline was achieved. However, the caffeine based MIP stationary phase did not possess specific selectivity to the template molecule, caffeine. MIP with specific selectivity to template molecule can only be prepared when interactions between template and monomer go together.