核苷酸、聚核苷酸和核酸猝灭Tb3+-钛铁试剂络合物荧光的机理研究

研究了核苷酸、聚核苷酸和核酸对Tb³⁺－钛铁试剂(TR)络合物的荧光碎灭机理,认为荧光猝灭过程是核苷酸、聚核苷酸和核酸分子中的磷酸基组分与TR竞争Tb³⁺离子,生成实验条件下无荧光的二元络合物的静态猝灭过程;用Tb³⁺－TR络合物荧光探针研究DNA嵌入剂和金属离子与DNA相互作用的实验结果说明这一机理是合理的.

Studies on the Mechanism of Fluorescence Quenching of Tb~(3+)-Tiron Complex by Nucleotides, Polynucleotides and Nucleic Acids

The mechanism of fluorescence quenching of Tb3+-Trion complex by nucleotides,polynucleotides and nucleic acids has been studied. It was considered that it is a static quenching which is based on the phosphate moieties of nucleotides, polynucleotides and nucleic acids conpeting the Tb3+ ion with Tiron and forming non-fluorescent binary complex under the optimal conditions. The experimental results of nucleic acids reacting with intercalating reagent of DNA or metal ion using Tb3+-Trion complex as a fluorescence probe indicated that the mechanism is reasonable.