| 羟基多环芳烃的电喷雾电离离子阱串联质谱检测 |
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| 引用本文： | 李雪,方小伟,李银萍,陈焕文.羟基多环芳烃的电喷雾电离离子阱串联质谱检测[J].高等学校化学学报,2013,34(8):1840-1844. |
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| 作者姓名： | 李雪 方小伟 李银萍 陈焕文 |
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| 作者单位： | 1. 上海大学环境与化学工程学院, 环境污染与健康研究所, 上海 200444;
2. 东华理工大学江西省质谱科学与仪器重点实验室, 南昌 330013 |
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| 基金项目： | 国家自然科学基金(批准号:21107066);国家重大科学仪器设备开发专项(批准号:2011YQ170067);上海高校青年教师培养资助计划和上海大学创新基金资助 |
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| 摘 要： | 采用电喷雾电离离子阱串联质谱检测了1-/2-羟基萘、 2-羟基芴、 2-/3-/4-/9-羟基菲、 6-羟基屈和3-羟基苯并a]芘等9种不同环数的羟基多环芳烃(OH-PAHs, 2~5环), 考察了碰撞诱导解离操作参数活化值Q和相对碰撞能量对羟基多环芳烃各单体碎片离子产率的影响. 通过优化活化值Q和相对碰撞能量, 得到了3-羟基苯并a]芘的碎片离子, 提高了1-羟基萘、 2-羟基芴、 3-/9-羟基菲和6-羟基屈碎片离子的产率, 并发现活化值Q是电喷雾电离离子阱串联质谱检测不同环数PAHs的关键参数.
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| 关 键 词： | 多环芳烃 生物标志物 羟基多环芳烃 离子阱串联质谱 碰撞诱导解离 |
| 收稿时间： | 2013-05-07 |
Detection of Hydroxylated Metabolites of Polycyclic Aromatic Hydrocarbons by Electrospray Ionization Ion Trap Tandem Mass Spectrometry |
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| LI Xue,FANG Xiao-Wei,LI Yin-Ping,CHEN Huan-Wen.Detection of Hydroxylated Metabolites of Polycyclic Aromatic Hydrocarbons by Electrospray Ionization Ion Trap Tandem Mass Spectrometry[J].Chemical Research In Chinese Universities,2013,34(8):1840-1844. |
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| Authors: | LI Xue FANG Xiao-Wei LI Yin-Ping CHEN Huan-Wen |
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| Institution: | 1. Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;
2. Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang 330013, China |
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| Abstract: | Hydroxylated metabolites of polycyclic aromatic hydrocarbons(OH-PAHs) are the mostly widely used biomarker of carcinogenic PAHs to assess health risk caused by exposure to PAHs. Among present strategies used for detecting OH-PAHs, electrospray ionization tandem mass spectrometry(ESI-MS2) shows advantages in both high sensitivity and selectivity; however, fragment ions cannot be obtained for some OH-PAHs(e.g. 3-OHBaP, 2-OHFlu). In this work, ESI-MS2 analysis of nine OH-PAHs(2- to 5-rings) was achieved with ion trap MS2(IT-MS2); by optimizing collision-induced dissociation conditions of activation Q(AQ) and normalized collision energy(NCE), 3-OHBaP and 2-OHFlu were effectively fragmented meanwhile fragmentation efficiencies of other seven OH-PAHs were also improved. AQ was proven to be the critical parameter for fragmenting OH-PAHs when IT-MS2 is used. |
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| Keywords: | Polycyclic aromatic hydrocarbon Biomarker Hydroxylated metabolite of polycyclic aromatic hydrocarbon Ion trap tandem mass spectrometry Collision-induced dissociation |
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