| 羟基磷灰石对牛血清白蛋白的吸附特性研究 |
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| 引用本文： | 殷钢,詹劲,刘铮,袁乃驹.羟基磷灰石对牛血清白蛋白的吸附特性研究[J].高等学校化学学报,2001,22(5):771-775. |
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| 作者姓名： | 殷钢 詹劲 刘铮 袁乃驹 |
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| 作者单位： | 清华大学化工系, |
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| 基金项目： | 国家自然科学基金! (批准号 :2 96760 2 2 )，北京市自然科学基金! (批准号 :2 992 0 16)资助 |
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| 摘 要： | 研究了羟基磷灰石表面电位随溶液PO4^3-浓度、Ca^2 浓度、离子强度和pH的变化规律；测定了不同操作条件下牛血清白蛋白在羟基磷灰石上的吸附容量；吸附等温线的测定结果表明该吸附属于Langmuir型；通过对该吸附过程的动力学研究，计算得到的表观活化能和吸附热数值较低，表明该吸附是物理吸附。
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| 关 键 词： | 羟基磷灰石 牛血清白蛋白 Zeta电位 吸附 动力学 分离 表观活化能 吸附热 物理吸附 |
| 文章编号： | 0251-0790（2001）05-0771-05 |
Characterization of the Adsorption of Bovine Serum Albumin on Hydroxylapatite |
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| YIN Gang,ZHAN Jin,LIU Zheng,YUAN Nai-ju.Characterization of the Adsorption of Bovine Serum Albumin on Hydroxylapatite[J].Chemical Research In Chinese Universities,2001,22(5):771-775. |
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| Authors: | YIN Gang ZHAN Jin LIU Zheng YUAN Nai-ju |
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| Abstract: | Experimental studies on the effects of the concentration of PO3－4 and Ca2＋, ion strength and pH of buffer on the polarity and magnitude of ζ-potential of hydroxylapatite(HAP) were carried out in present study to characterize the surface physicochemical properties of hydroxylapatite. Adsorption of bovine serum albumin(BSA) on HAP was carried at different conditions in terms of pH, ionic strength, and the concentration of PO3－4 to establish the knowledge of adsorption mechanism. A Langmuir adsorption isotherm was obtained and the apparent activation energy and adsorption heat were interpreted. The low magnitude of the apparent activation energy and the adsorption heat indicated the fast adsorption of BSA on hydroxylapatite was a physical adsorption in nature. |
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| Keywords: | Hydroxylapatite Bovine serum albumin \%Zeta\% potential Adsorption Kinetics |
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