二茂铁标记DNA电化学探针的研制及性质研究

以乙基－（3－二甲基丙基）碳化二亚胺盐酸(EDC)为偶联活化剂，利用缩合反应分别将电化学活性物质氨基二茂铁（Aminoferrocene,AFC)和苯基二茂铁（Ferrocencearboxadehyde,FCA)成功地标记在变性小牛胸腺DNBA片段上，制备成二茂铁标记DNA探针，分别采用电化学，紫外，红外光谱等方法研究了二茂铁标记DAN探针的性质，计算了二茂铁的标记效率，变性小牛胸腺DNA的反应效率以及二茂铁化合物与变性小牛胸腺DNA的反应比率，实验表明，氨基二茂铁和醛基二茂铁与DNA上的磷酸基是以1：1比率进行的反应，该标记反应不影响DNA链碱基的紫外吸收，二茂铁标记DNA探针在石墨电极上有良好的电化学影响，将制备好的二茂铁标记DNA电化学探置于冰箱中冷冻（温度低于－15度）保存3个月后用于测定，峰电流仅下降1．2％。

Studies on the Synthesis and Characters of Ferrocene Labeled DNA Probe

The preparation and characters of a ferrocene labeled DNA probe was studied. Ferrocene was labeled onto a denatured calf thymus DNA by EDC. The electrochemical character, UV spectrum and IR spectrum of the ferrocene labeled probe were discussed. It was suggested that the ferrocene be labeled on the phosphoric group of calf thymus DNA. The probe synthesis was simple, quick and of efficiency. It made bases for the application of electrochemical DNA probe to DNA detection, damage and protection.