| 不同表面性质聚电解质多层膜的制备及蛋白质吸附和血液相容性能 |
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| 引用本文： | 彭采宇,仝维滗,韩宝三,王兆海,高长有,沈家骢.不同表面性质聚电解质多层膜的制备及蛋白质吸附和血液相容性能[J].高等学校化学学报,2009,30(11):2311-2318. |
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| 作者姓名： | 彭采宇 仝维滗 韩宝三 王兆海 高长有 沈家骢 |
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| 作者单位： | 1. 浙江大学高分子科学与工程学系,教育部高分子合成与功能构造重点实验室,杭州,310027
2. 上海交通大学瑞金医院器官移植中心,上海,210025 |
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| 基金项目： | 国家自然科学基金，国家"九七三"计划 |
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| 摘 要： | 利用层层自组装方法制备了聚烯丙基铵盐酸盐(PAH)/聚苯乙烯磺酸钠(PSS)多层膜. 通过吸附或共价偶联, 在多层膜表面修饰了聚乙二醇(PEG)、牛血清白蛋白(BSA)或肝素, 通过石英晶体微天平(QCM)、椭圆偏振光谱和原子力显微镜(AFM)研究了多层膜的表面形貌及修饰方法对各种蛋白的吸附性能. 经修饰后的多层膜较基底膜的厚度均有所增大; 最外层经修饰后的多层膜吸附的BSA、纤维蛋白原及血浆蛋白的量较未修饰多层膜均有所减少. 采用SEM观察了血小板在多层膜上的黏附情况和形态变化, 计算了血小板的黏附率. 比较各多层膜的凝血酶原时间(PT), 发现修饰后的多层膜的凝血酶原时间均有所延长, 但各组间无显著性差异.
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| 关 键 词： | 聚电解质多层膜 肝素 白蛋白 聚乙二醇 血液相容性 |
| 收稿时间： | 2009-02-05 |
Preparation,Protein Adsorption and Blood Compatibility of Polyelectrolyte Multilayers with Different Surface Properties |
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| PENG Cai-Yu,TONG Wei-Jun,HAN Bao-San,WANG Zhao-Hai,GAO Chang-You,SHEN Jia-Cong.Preparation,Protein Adsorption and Blood Compatibility of Polyelectrolyte Multilayers with Different Surface Properties[J].Chemical Research In Chinese Universities,2009,30(11):2311-2318. |
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| Authors: | PENG Cai-Yu TONG Wei-Jun HAN Bao-San WANG Zhao-Hai GAO Chang-You SHEN Jia-Cong |
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| Institution: | PENG Cai-Yu1,TONG Wei-Jun1,HAN Bao-San2,WANG Zhao-Hai2,GAO Chang-You1*,SHEN Jia-Cong1(1.Key Laboratory of Macromolecular Synthesis and Functionalization,Ministry of Education and Department of Polymer Science and Engineering,Zhejiang University,Hangzhou 310027,China,2.Organ Transplantation Center of Ruijin Hospital Affiliated to Medical College of Shanghai Jiaotong University,Shanghai 200025,China) |
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| Abstract: | Poly(styrene sulfonate)/poly(allylamine hydrochloride) multilayers were assembled on silicone wafers via the technique of layer-by-layer assembly.Their surfaces were further modified either physically or chemically with bovine serum albumin(BSA),heparin and polyethylene glycol(PEG).Protein adsorption on these surfaces was investigated by quartz crystal microbalance(QCM),ellipsometry and atomic force microscopy(AFM).The dynamic adsorption process of BSA on these multilayers was monitored by QCM,revealing tha... |
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| Keywords: | Polyelectrolyte multilayer Heparin Albumin Polyethylene glycol Blood compatibility |
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