新型杂环三氮烯荧光试剂1,8-双(2-苯并噻唑重氮氨基)萘的合成及其分析应用

将1,8-萘二胺和苯并噻唑类试剂结合, 并引入杂环三氮烯结构, 合成了新荧光试剂1,8-双(2-苯并噻唑重氮氨基)萘(BBTANP), 其结构经红外光谱、核磁共振谱和元素分析证实. 研究结果表明, 在碱性介质中, 该试剂与Cu(Ⅱ)形成1∶1的络合物, 并在λex/λem=362 nm/459 nm处产生荧光增强作用. 据此建立了BBTANP测定Cu(Ⅱ)的新型荧光分析法, 该方法的线性范围为8.0×10-7—1.0×10-5 mol /L, 检测限为1.3×10-7 mol/L. 将其应用于水样中Cu(Ⅱ)的测定, 结果令人满意.

Synthesis and Analytical Application of a New Heterocyclic Triazene Fluorescent Reagent 1,8-Bis(2-benthiazolydiazoamino)naphthalene

With the aid of combining 1,8-naphthalene with benthiazole through bis-triazene group, a new fluorescent reagent 1,8-bis(2-benthiazolydiazoamino)naphthalene(BBTANP) was synthesized and confirmed by means of elemental analysis, 1H NMR and infrared spectra. A chelate complex was formed by the reaction between BBTANP and Cu(Ⅱ) in a weak basic medium, which resulted in fluorescence enhancement. The possible response mechanism of BBTANP to Cu(Ⅱ) was discussed. The reaction showed a high selectivity and a high sensitivity for Cu(Ⅱ). A new spectrofluorimetric method for the determination of trace Cu(Ⅱ) with this reagent was developed. The excitation and emission wavelengths of BBTANP/Cu(Ⅱ) system were 362 and 459 nm in a basic medium, respectively. Under the optimal conditions, a linear response to copper was obtained in the concentration range from 8.0×10-7 to 1.0×10-5 mol/L, and the detection limit was 1.3×10-7 mol/L. The method is selective, sensitive and simple. It has been used to determine trace copper in lake water with satisfactory results.