首页 | 本学科首页   官方微博 | 高级检索  
     检索      

不同电荷态泛素蛋白离子的193 nm紫外光解离质谱
引用本文:周敏,石莹莹,李树奇,张凯林,崔永亮,张森,张先燚,孔祥蕾.不同电荷态泛素蛋白离子的193 nm紫外光解离质谱[J].高等学校化学学报,2021,42(8):2436.
作者姓名:周敏  石莹莹  李树奇  张凯林  崔永亮  张森  张先燚  孔祥蕾
作者单位:安徽师范大学物理与电子信息学院,芜湖241000;南开大学化学学院元素有机化学国家重点实验室,天津300071;南开大学化学学院元素有机化学国家重点实验室,天津300071;南开大学化学学院元素有机化学国家重点实验室,天津300071;天津大学精密仪器与光电子工程学院,天津300072;安徽师范大学物理与电子信息学院,芜湖241000;南开大学化学学院元素有机化学国家重点实验室,天津300071;南开大学化学科学与工程创新中心,天津300071
基金项目:国家自然科学基金(21627801)
摘    要:将193 nm激光与傅里叶变换离子回旋共振(FT-ICR)质谱仪结合, 研究了不同价态的泛素蛋白离子的紫外光解离质谱. 结果表明, 在光解离过程中向分析池内引入适量的碰撞气, 不仅能增加母体离子的裂解率, 也能提高碎片离子的捕获效率. 相对于碰撞辅助解离(CAD)中产生的b和y离子, 紫外光解离(UVPD)方法能够产生更为丰富的不同种类碎片离子. 其中, 对于+11价泛素离子, 蛋白质序列的覆盖率接近80%, 远高于对应的CAD实验结果. 与已往报道不同的是, 裂解覆盖率呈现出较强的电荷相关性. 因此, 如何进一步提高较低电荷态的蛋白离子的解离效率和序列覆盖率还需深入研究.

关 键 词:自顶向下  紫外光解离  碰撞活化解离  泛素  电荷态
收稿时间:2021-01-04

193 nm UV Photodissociation Mass Spectrometry for Ubiquitin Ions with Different Charge States
ZHOU Min,SHI Yingying,LI Shuqi,ZHANG Kailin,CUI Yongliang,ZHANG Sen,ZHANG Xianyi,KONG Xianglei.193 nm UV Photodissociation Mass Spectrometry for Ubiquitin Ions with Different Charge States[J].Chemical Research In Chinese Universities,2021,42(8):2436.
Authors:ZHOU Min  SHI Yingying  LI Shuqi  ZHANG Kailin  CUI Yongliang  ZHANG Sen  ZHANG Xianyi  KONG Xianglei
Institution:1.School of Physics and Electronic Information,Anhui Normal University,Wuhu 241000,China;2.State Key Laboratory and Institute of Elemento?Organic Chemistry,Nankai University,Tianjin 300071,China;3.School of Precision Instrument and Opto?Electronics Engineering,Tianjin University,Tianjin 300072,China;4.Innovation Center of Chemical Science and Engineering,Nankai University,Tianjin 300071,China
Abstract:In recent years, the application of ultraviolet photodissociation(UVPD) in protein top-down mass spectrometry has attracted more and more attentions. In this study, the UVPD mass spectra of ubiquitin ions with different charge states were studied by combining a 193 nm laser with a fourier transform ion cyclotron resonance(FT-ICR) mass spectrometer. Results showed that the efficiency of ion fragmentation and trapping could be improved by introducing an appropriate amount of collision gas into the analysis cell during photolysis. Compared with the pure b, y ions produced in collision-activated dissociation(CAD) experiments, UVPD method produced more abundant fragment ions in both intensity and diversity. For +11 ubiquitin ions, the protein sequence coverage was close to 80%, which was much higher than the corresponding experimental results of CAD. However, different from the previous reports, the fragmentation coverage still showed a correlation with the charge state of the ions. For the lower charged protein ions, further research for a better dissociation efficiency and sequence coverage is still needed very much.
Keywords:Top-down  Ultraviolet photodissociation  Collision-activated dissociation  Ubiquitin  Charge state  
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《高等学校化学学报》浏览原始摘要信息
点击此处可从《高等学校化学学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号