4-氨基吡啶分子模板聚合物选择性识别及结合性质的研究

以4-氨基吡啶为模板分子制备了对4-氨基吡啶具有特异选择性的模板聚合物.制得的棒状聚合物经研磨、过筛后,采用平衡结合方法评价了该模板聚合物的结合特性.Scatchard分析表明,在所研究的浓度范围内,聚合物中形成了两类不同的结合位点,这两类结合位点的离解常数采用多点结合模型计算得到的值分别为6.0μmol/L和1.2mmol/L.底物选择性表明,与其它结构相似的分子相比,该聚合物对模板分子4-氨基吡啶显示了很强的结合能力.₁HNMR研究及4-氨基吡啶共振结构分析表明,模板分子与聚合物中的结合位点之间的作用为离子作用.

Studies on the Binding Nature in Molecularly Imprinted Polymer Selective for 4-Aminopyridine

A molecularly imprinted polymer was prepared using 4-aminopyridine as template. The bulk polymer obtained was ground up, sieved and investigated in equilibrium binding experiments to evaluate the binding characteristics of the 4-aminopyridine-imprinted polymer. Scatchard analysis showed that two classes of binding sites were produced in the polymer matrix and their dissociation constants were calculated to be 6.0 μmol/L and 1.2 mmol/L respectively, by utilizing the model of multiple independent classes of binding sites. The substrate selectivity of the polymer was investigated. The results showed that the imprinted polymer exhibited much higher affinity for 4-aminopyridine among the tested compounds. 1H NMR studies and the analysis of the resonance structure of 4-aminopyridine indicated that the selective binding interaction between the template and binding sites in the polymer arose from their ionic interaction.