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| 量子点标记天花粉蛋白的研究 | |
| 引用本文： | 张春阳,马辉,丁尧,金雷,陈瓞延,苗琦,聂书明.量子点标记天花粉蛋白的研究[J].高等学校化学学报,2001,22(1):34-37. |
| 作者姓名： | 张春阳 马辉 丁尧 金雷 陈瓞延 苗琦 聂书明 |
| 作者单位： | 1. 清华大学物理系 2. 中国科学院生物物理研究所 3. 印第安那大学化学系, |
| 基金项目： | 国家基金委海外青年学者合作基金!(批准号 :19992 840 8)，清华大学985基金项目资助 |
| 摘 要： | 将半导体量子点应用于记天花粉蛋白，研究了量子占标记天花粉蛋白的吸收光谱、荧光光谱和酶活性变化，与游离的QDs 相比，QDs-TCS的吸收光谱在400－600nm范围内不发生明显变化，QDs-TCS的发射光普发生蓝移，但发射半宽不变，标记上QDs后TCS的酶活性没有发生改变，利用双光子激发扫描荧光显微镜和激光共取焦显微镜同时观察QDs-TCS在人绒癌细胞内的分布发现，QDs-TCS能够跨膜进入细胞，并在细胸核周围呈聚集分布。 |
| 关 键 词： | 天花粉蛋白 酶活性 半导体量子点 标记 中药研究 生物荧光探针 |
| 文章编号： | 0251-0790(2001)01-0034-04 |
Studies on Quantum Dots-labeled Trichosanthin |
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| ZHANG Chun-yang,MA Hui,DING Yao,JIN Lei,CHEN Die-yan,MIAO Qi,NIE Shu-Ming.Studies on Quantum Dots-labeled Trichosanthin[J].Chemical Research In Chinese Universities,2001,22(1):34-37. | |
| Authors: | ZHANG Chun-yang MA Hui DING Yao JIN Lei CHEN Die-yan MIAO Qi NIE Shu-Ming |
| Abstract: | Semiconductor quantum dots were used for the first time to label trichosanthin. The absorption spectrum, fluorescent spectrum and enzymatic activity of quantum dots-labeled trichosanthin were studied in this paper. The absorbane of QDs-TCS varied to a less extent with wavelength in the range of 400\_600 nm. The fluorescent spectrum of QDs-TCS underwent a blue-shift in the emission peak, but the intrinsic spectral width was unchanged. No changes were found in the enzymatic activity of QDs-TCS in comparison with trichosanthin. Distribution of QDs-TCS within human choriocarcinoma cells was simultaneously observed with two-photon laser scanning microscopy and confocal laser scanning microscopy. The results indicated that QDs-TCS could enter the cytoplasm across the membrane and aggregated around the nuleolus. |
| Keywords: | Quantum dots Trichosanthin Enzymatic activevity |
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