稀水溶液中聚乙烯吡咯烷酮构象行为的荧光研究

以苊烯(ACE)为荧光标记物,8-苯胺基-萘磺酸(ANS)为荧光探针,利用荧光各向异性、荧光猝灭以及非辐射能量转移等静态荧光技术研究了聚乙烯吡咯烷酮(PVP)在稀水溶液中的构象行为.结果表明,PVP在稀水溶液中呈松散自由线团构象,这种构象不随溶液pH的变化而显著变化.不同于其它水溶性高分子,PVP有很强的氢键形成能力,易与具有活泼氢的其它高分子或小分子相结合,并易被金属氧化物类吸附剂吸附.因此PVP可被用来修饰某些具有疏水结构的水溶性高分子.PVP水溶液在pH=7以上相当稳定,放置1周性质没有显著变化.

Fluorescence Studies of the Conformational Behavior of Poly(vinyl-pyrro lydone) in Dilute Aqueous Solution

Fluorescence anisotropy, fluorescence quenching and non-radiative energy transfer studies \%via\% the acenaphthylene(ACE) label and 8-anilino-naphthylene sulfornic acid(ANS) probe have demonstrated that poly(vinyl pyrrolydone)(PVP) adopts loose coiled conformation within a wide pH range. Unlike other water-soluble polymers, PVP tends to combine other polymers and/or small molecules with active hydrogen in their structures. This property may be employed to modify the conformational behavior of some water-soluble polymers with hydrophobic microstructures. The nature of the combination may be attributed to hydrogen bond formation, which makes it be easily adsorbed by metal oxide, including SiO 2, Fe 3O 4 \%etc\%. Fluorescence studies have also indicated that the aqueous solution of PVP is stable at least within one week if the pH of the solution is greater than 7.