载细胞海藻酸钠/壳聚糖微胶囊的化学破囊方法研究

以海藻酸钠-壳聚糖-海藻酸钠微胶囊(简称ACA微胶囊)为研究体系,建立了一种生理条件下ACA微胶囊的化学破囊方法,破囊过程充分考虑了对囊内生物物质活性的保持.以微生物细胞PichiapastorisGS115和动物细胞L929为模型,以NaHCO3和Na3C6H5O7·2H2O为破囊液基本组分,考察了破囊液对ACA微胶囊的破囊效果及破囊过程对囊内细胞活性的影响.结果表明,破囊操作可在30s内完成,破囊率为100%,微胶囊膜完全溶解,破囊后细胞存活率在85%以上.

Chemical Method of Breaking the Cell-loaded Sodium Alginate/Chitosan Microcapsules

A novel chemical method to break and dissolve cell-loaded microcapsules under physiological condition was established, especially aiming at alginate/chitosan/alginate(ACA) microcapsules. The microcapsule-broken process was designed to proceed under an approximately physiological condition for the consideration of maintaining the cytoactive. The microbial cell(Pichia pastoris GS115) and animal cell(L929) were chosen as the model cells, and the microcapsule-broken solution(MBS) was basically composed of NaHCO3 and Na3C6H5O7·2H2O. The effects of MBS on the breaking of microcapsules and on the cytoactive were thoroughly investigated. Moreover, the mechanism of the breaking of microcapsules was analyzed. It was demonstrated that the microcapsules could be broken within 30 s, the breaking ratio of the microcapsule was 100%, and the fragments of the microcapsules was dissolved completely. It was also determined that the survival rate of cells was above 85%. Thus, the novel method will make it possible to quantitatively analyze the growth of cells in microcapsules, to examine the morphology and function of cells, to recover the cells and to purify metabolic products, which is always difficult to realize at present. Therefore, the chemical method of breaking and dissolving cell-loaded microcapsules would be the important component of microencapsulation technology applied to the cell culture and cell transplantation.