| 现场紫外光谱及圆二色谱研究电化学还原反应诱导微过氧化物酶-11构象的转变 |
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| 引用本文： | 姜秀娥,张哲泠,黄卫民,刘小强,汪尔康,董绍俊.现场紫外光谱及圆二色谱研究电化学还原反应诱导微过氧化物酶-11构象的转变[J].高等学校化学学报,2005,26(2):321-325. |
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| 作者姓名： | 姜秀娥 张哲泠 黄卫民 刘小强 汪尔康 董绍俊 |
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| 作者单位： | 中国科学院长春应用化学研究所, 电分析化学国家重点试验室, 长春 130022 |
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| 摘 要： | 采用现场紫外光谱及圆二色谱电化学方法研究了微过氧化物酶-11的电化学还原过程.同时应用奇异值分解最小二乘法和双对数法对所得光谱数据进行处理.研究发现,电化学还原过程诱导微过氧化物酶-11的构象由无规卷曲向α螺旋转变,这为进一步理解生物电子传递过程与生物分子构象转变机理提供了基础信息.
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| 关 键 词： | 微过氧化物酶-11 光谱电化学 电子传递 构象变化 |
| 文章编号： | 0251-0790(2005)02-0321-05 |
| 收稿时间： | 2004-02-27 |
In-situ UV and CD Spectroelectrochemistry Investigation of the Electrochemical Reduction Reaction Inducing Conformational Transition of Microperoxidase-11 |
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| JIANG Xiu-E,ZHANG Zhe-Ling,HUANG Wei-Min,LIU Xiao-qiang,WANG Er-Kang,DONG Shao-Jun.In-situ UV and CD Spectroelectrochemistry Investigation of the Electrochemical Reduction Reaction Inducing Conformational Transition of Microperoxidase-11[J].Chemical Research In Chinese Universities,2005,26(2):321-325. |
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| Authors: | JIANG Xiu-E ZHANG Zhe-Ling HUANG Wei-Min LIU Xiao-qiang WANG Er-Kang DONG Shao-Jun |
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| Institution: | State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China |
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| Abstract: | Electrochemical reduction process of microperoxidase-11 was investigated by in-situ UV-Vis and CD spectroelectrochemistry methods with a long optical path thin layer cell. The spectra obtained were analyzed by singular value decomposition least square and double logarithmic analysis methods. The results showed that the electrochemical reduction induced the conformational transition of MP-11 from random coil to α-helix and provided an important information for understanding biological electron transfer mechanism coupled with conformational transitions. |
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| Keywords: | Microperoxidase-11 Spectroelectrochemistry Electron transfer Conformation change |
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