脉冲电沉积制备HA/ZrO2纳米复合涂层的稳定性及生物相容性评价

采用脉冲电化学沉积法成功地在生物医用钛金属表面制备出均匀的纳米HA/ZrO₂复合涂层. 通过热处理提高涂层的致密性, 同时保留涂层的微纳结构. 考察了热处理后复合涂层的成分、形貌、生物相容性及生理稳定性. X射线衍射分析表明, 复合涂层成分为HA和ZrO₂. 扫描电镜观察发现, 热处理后复合涂层的致密性有所提高. 研究发现, ZrO₂的加入大大降低了HA/ZrO₂复合涂层中钙离子的释放速度, 提高了HA/ZrO₂复合涂层的生理稳定性. 纳米划痕实验结果表明, HA/ZrO₂复合涂层具有较好的结合强度. 通过培养成骨细胞考察了复合涂层的生物相容性. Alamar Blue检测表明, HA/ZrO₂复合涂层表面细胞黏附及增殖能力较好. ALP检测发现, 热处理后HA/ZrO₂复合涂层表面的细胞分化能力较强. 综合细胞培养结果显示, HA/ZrO₂复合涂层有较好的生物相容性.

Investigation of the Stability and Biocompatibility of HA/ZrO_2 Nanocomposite Coatings Prepared by Pulsed Electrochemical Deposition

The dense and uniform HA/ZrO2 composite coatings were fabricated on titanium substrates by pulsed electrochemical deposition. The composition, morphologies, biocompatibility and physiological stability of the composite coatings were studied. X-ray diffraction showed Ca8(PO4)4(HPO4)2·5H2O in the coating was converted into hydroxylapatite(HA) and the coating was composed of HA and ZrO2 after heat treatments. Scanning electron microscopy(SEM) indicated that ZrO2 was uniformly distributed in the coatings and the coatings became dense after heat treatments. Atomic absorption spectrometry analysis was used to measure Ca2+ release rate of the coatings immersed in PBS. It was found that the Ca2+ release rate of HA/ZrO2 was lower than that of pure HA coatings. Nanoscratching testing revealed that HA/ZrO2 coatings had better interfacial bonding strength than that of pure HA coatings. Osteoblasts were cultured on the coatings to evaluated the biocompatibility of coatings. The results of the Alamar Blue and ALP test indicated that the composite coatings could favor the proliferation and differentiation of the osteoblasts. In summary, the as-prepared nanocomposite coatings have good biocompatibility.