负载高密度乙肝检测探针磁珠的制备及性能

通过化学键连接的方法制备出表面偶联乙肝抗体的磁性复合微球(亦称"乙肝抗原检测免疫磁珠"),并用于乙肝检测.结果表明,该方法具有较好的检测效果.通过优化得到氨基磁性复合微球的氨基含量为2.71mmoL/g,单位磁珠抗体偶联量为108.36μg/mg,偶联效率为77.40%的负载高密度乙肝检测探针的磁珠.采用类"双抗原夹心...

Preparation and Properties of High-Density HBsAg-Conjugated Magnetic Probes

The preparation of magnetic beads loaded with high-density Hepatitis B Surface Antigen (HBsAg) detection probes was introduced in this paper, and the properties of the beads for HBsAg detection were also studied. The immunomagnetic beads coupled with HBsAb were firstly prepared by chemical bond. Then, immunomagnetic beads were used for detecting HBsAg and were obtained a favorable effect. In order to improve the accuracy and sensitivity of detection, the preparation process of immunomagnetic beads was optimized, including amination and coupling process. Through the optimization, the amino content of magnetic beads was 2.71mmol/g, unit magnetic beads bonded 108.36 ug/mg antibodies and the coupling efficiency was 77.40%. The activity of HBsAb on magnetic beads was detected by the method similar to Enzyme-Linked Immunosorbent Assay (ELISA). Compared with common ELISA technology, magnetic beads loaded with high-density HBsAb have higher sensitivity.