温度及药物诱导肿瘤细胞凋亡的实验观察

将微量热法和透射电镜技术相结合，研究了温度对人卵巢癌细胞代谢的影响，不同温度下癌细胞超微结构的变化和加抗肿瘤药物（嘧福绿）的协同作用，从宏观到微观，深入探讨了热与化序协同作用对肿瘤细胞杀伤作用的机理，实验结果表明，在高温下细胞代谢降低，肿瘤细胞的形态发生一系列恶性变化，且高热可能诱导肿瘤细胞的凋亡；热疗加化疗的协同作用则能加速肿瘤细胞的凋亡，使细胞的能量代谢更低，随着作用时间的延长，最终导致细胞死亡。

Experimentation on Induction of Tumor Cell Apoptosis by Temperature and Anti-tumor Medicine

Techniques from microcalorimetry and transmission electron microscopy(TEM) were employed to investigate the metabolic effects and the ultrastructural changes of human body carcinoma of ovary cell line(COC 1) at different temperatures with and without synergetic effects of anti tumor medicine(HCFU). The mechanism of hyperthemia and chemotherapy in the treatment of the tumor cells was explored at both macroscopic and microscopic levels. The results showed that the metabolic activity of the tumor cells was reduced at high temperature and the cell morphology changed malignantly. High temperature might induce apoptosis of the tumor cells; and the combined effects of hyperthemia and chemotherapy could speed up the apoptosis and reduce the metabolic power of the tumor cells. A sustained longer time action could lead to ultimate death of the tumor cells.