| 一种新的玉米抗氧化肽的制备与结构表征 |
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| 引用本文： | 徐力,李相鲁,吴晓霞,王华,侯瑞珍,黄宜兵,张学忠.一种新的玉米抗氧化肽的制备与结构表征[J].高等学校化学学报,2004,25(3):466-469. |
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| 作者姓名： | 徐力 李相鲁 吴晓霞 王华 侯瑞珍 黄宜兵 张学忠 |
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| 作者单位： | 1. 吉林大学分子酶学工程教育部重点实验室, 长春 130023;
2. 吉林大学中日联谊医院, 长春 130033;
3. 长春工业大学化工学院, 长春 130012 |
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| 基金项目： | 国家“十五”科技攻关计划 (批准号 :2 0 0 1BA960 C)资助 |
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| 摘 要： | 用酶法在受控条件下降解玉米醇溶蛋白,对酶解产物进行分离纯化,获得一种新的抗氧化肽;对其一级结构进行了表征,氨基酸组成和顺序为Leu-Asp-Tyr-Glu;从结构上分析了其可能的抗氧化机制.
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| 关 键 词： | 玉米肽 酶水解 抗氧化性 层析 质谱 |
| 文章编号： | 0251-0790(2004)03-0466-04 |
| 收稿时间： | 2003-02-17 |
| 修稿时间： | 2003年2月17日 |
Preparation and Structural Characterization of a New Corn Anti-oxidative Peptide |
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| XU Li ,LI Xiang Lu ,WU Xiao Xia ,WANG Hua ,HOU Rui Zhen ,HUANG Yi Bing ,ZHANG Xue Zhong.Preparation and Structural Characterization of a New Corn Anti-oxidative Peptide[J].Chemical Research In Chinese Universities,2004,25(3):466-469. |
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| Authors: | XU Li LI Xiang Lu WU Xiao Xia WANG Hua HOU Rui Zhen HUANG Yi Bing ZHANG Xue Zhong |
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| Institution: | 1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130023, China;
2. China Japan Union Hospital of Jilin University, Changchun 130033, China;
3. College of Chemical Engineering, Changchun University of Technology. Changchun 130012, China |
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| Abstract: | A kind of new corn anti oxidative peptide was prepared via protease hydrolysis of zein catalyzed by alcalase, an alkaline protease under a certain condition, followed by isolation and purification. The hydrolysis time was fixed on the basis of the dependence of antioxidative activity on hydrolysis degree. It was found that the hydrolysis for 4 h gave the highest antioxidative activity. The isolation and purification steps include ultrafiltration with a membrane of cutoff M w 1 000, successive chromatography on Sephadex G 15, Amberlite CG 120(Na) ion exchange, Sephadex G 10 and preparative HPLC columns. The amino acid sequence of the antioxidative peptide was characterized to be Leu Asp Tyr Glu by amino acid analysis and electrospray ion trap mass spectrometry. We also discussed the possible anti oxidative mechanism of the antioxidative peptide on the basis of its structure. |
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| Keywords: | Corn peptide Enzymatic hydrolysis Antioxidative activity Chromatography Mass spectrometry |
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